ProtParam
User-provided sequence:
10 20 30 40 50 60
MVSLDKAVIA RLRKGGEEFE VLVDPYLARD LKEGKEVNFE DLLAAEEVFK DAKKGERASV
70 80 90 100 110 120
DELRKIFGTD DVFEIARKII LEGEVQITAE QRREMLEAKR KQIINFISRN TIDPRTNAPH
130 140 150 160 170 180
PPSRIERALE EAKVHIDIFK SVEAQVKDIV KALKPILPLK FEEMEIAIKI PPEHTGRAIS
190 200 210 220 230
ALYNFGGVTR EEWQRDGSWI CVMRIPSGMY GDLMDLLGKV AKGEALTKVL RRIG
References and
documentation are available.
Number of amino acids: 234
Molecular weight: 26564.86
Theoretical pI: 6.39
Total number of negatively charged residues (Asp + Glu): 41
Total number of positively charged residues (Arg + Lys): 40
Atomic composition:
Carbon C 1186
Hydrogen H 1938
Nitrogen N 330
Oxygen O 345
Sulfur S 7
Formula: C
1186H
1938N
330O
345S
7
Total number of atoms: 3806
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 15470
Abs 0.1% (=1 g/l) 0.582, assuming all pairs of Cys residues form cystines
Ext. coefficient 15470
Abs 0.1% (=1 g/l) 0.582, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 27.39
This classifies the protein as stable.
Aliphatic index: 99.62
Grand average of hydropathicity (GRAVY): -0.312