ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVSMPHWPKP IGLAPADLVC DMIPGRMRQL LDRLGNPEKK LPPVIHIAGT NGKGSTISFI 

        70         80         90        100        110        120 
RYILNSAGLK THIYTSPHLV EFNERVVLAG KRISDQYLHE VIEICREASE GIPVTFFEGT 

       130        140        150        160        170        180 
TAAILLAFSK VDADIVLVET GVGGRLDATN VVHPILNIIT SISLEHTEAL GNTVELIAGE 

       190        200        210        220        230        240 
KAGIMKENVT CVIAPQDQES IMNVLEYHAI KKNVPLYRGG IDWFCERKGD SMLFKSSVDE 

       250        260        270        280        290        300 
LLFPLPSLRG EHQIINAGNA IAACSILSGK FGYNIDYENI VQGLKEAYWP ARLEKITTGF 

       310        320        330        340        350        360 
LSKMVPQLWD LFLDGAHNPA GGEMLSRWIQ NNELGDLYII IGMTREKDSA VFLNYLKPYI 

       370        380        390        400        410        420 
KFLSSVCIKS EPRAQTAAEL LQTALSLGIK AVAAKTLADA ISKILEVGDC NRKSTILICG 

       430 
SLYLAGDVLL ENSME 

References and documentation are available.

Number of amino acids: 435

Molecular weight: 47704.41

Theoretical pI: 5.75

Amino acid composition: 
Ala (A)  35	  8.0%
Arg (R)  16	  3.7%
Asn (N)  20	  4.6%
Asp (D)  19	  4.4%
Cys (C)   8	  1.8%
Gln (Q)  10	  2.3%
Glu (E)  30	  6.9%
Gly (G)  34	  7.8%
His (H)  10	  2.3%
Ile (I)  42	  9.7%
Leu (L)  50	 11.5%
Lys (K)  25	  5.7%
Met (M)  11	  2.5%
Phe (F)  13	  3.0%
Pro (P)  20	  4.6%
Ser (S)  28	  6.4%
Thr (T)  20	  4.6%
Trp (W)   5	  1.1%
Tyr (Y)  12	  2.8%
Val (V)  27	  6.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 49
Total number of positively charged residues (Arg + Lys): 41

Atomic composition:

Carbon      C	      2145
Hydrogen    H	      3435
Nitrogen    N	       563
Oxygen      O	       624
Sulfur      S	        19

Formula: C2145H3435N563O624S19
Total number of atoms: 6786

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    45880
Abs 0.1% (=1 g/l)   0.962, assuming all pairs of Cys residues form cystines


Ext. coefficient    45380
Abs 0.1% (=1 g/l)   0.951, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 28.49
This classifies the protein as stable.



Aliphatic index: 108.53

Grand average of hydropathicity (GRAVY): 0.121