ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVSQFAIEVR NLTYKFKESS DPSVVDINLQ IPWNTRSLVV GANGAGKSTL LKLLSGKHLC 

        70         80         90        100        110        120 
LDGKILVNGL DPFSPLSMNQ VDDDESVEDS TNYQTTTYLG TEWCHMSIIN RDIGVLELLK 

       130        140        150        160        170        180 
SIGFDHFRER GERLVRILDI DVRWRMHRLS DGQKRRVQLA MGLLKPWRVL LLDEVTVDLD 

       190        200        210        220        230        240 
VIARARLLEF LKWETETRRC SVVYATHIFD GLAKWPNQVY HMKSGKIVDN LDYQKDVEFS 

       250        260        270        280 
EVVNAKVNGQ VAFENDNNKV VISKVNSLHP LALEWLKRDN QIPDKEIGI 

References and documentation are available.

Number of amino acids: 289

Molecular weight: 33089.98

Theoretical pI: 6.19

Amino acid composition: 
Ala (A)  11	  3.8%
Arg (R)  18	  6.2%
Asn (N)  17	  5.9%
Asp (D)  23	  8.0%
Cys (C)   3	  1.0%
Gln (Q)  10	  3.5%
Glu (E)  17	  5.9%
Gly (G)  16	  5.5%
His (H)   7	  2.4%
Ile (I)  17	  5.9%
Leu (L)  35	 12.1%
Lys (K)  19	  6.6%
Met (M)   6	  2.1%
Phe (F)   9	  3.1%
Pro (P)   8	  2.8%
Ser (S)  19	  6.6%
Thr (T)  12	  4.2%
Trp (W)   7	  2.4%
Tyr (Y)   6	  2.1%
Val (V)  29	 10.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 40
Total number of positively charged residues (Arg + Lys): 37

Atomic composition:

Carbon      C	      1477
Hydrogen    H	      2356
Nitrogen    N	       410
Oxygen      O	       434
Sulfur      S	         9

Formula: C1477H2356N410O434S9
Total number of atoms: 4686

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    47565
Abs 0.1% (=1 g/l)   1.437, assuming all pairs of Cys residues form cystines


Ext. coefficient    47440
Abs 0.1% (=1 g/l)   1.434, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 27.15
This classifies the protein as stable.



Aliphatic index: 103.08

Grand average of hydropathicity (GRAVY): -0.255