ProtParam
User-provided sequence:
10 20 30 40 50 60
MVSTHNRDKP WDTDDIDKWK IEEFKEEDNA SGQPFAEESS FMTLFPKYRE SYLKTIWNDV
70 80 90 100 110 120
TRALDKHNIA CVLDLVEGSM TVKTTRKTYD PAIILKARDL IKLLARSVPF PQAVKILQDD
130 140 150 160 170 180
MACDVIKIGN FVTNKERFVK RRQRLVGPNG NTLKALELLT KCYILVQGNT VSAMGPFKGL
190 200 210 220 230 240
KEVRRVVEDC MKNIHPIYHI KELMIKRELA KRPELANEDW SRFLPMFKKR NVARKKPKKI
250 260 270 280 290 300
RNVEKKVYTP FPPAQLPRKV DLEIESGEYF LSKREKQMKK LNEQKEKQME REIERQEERA
310
KDFIAPEEEA YKPNQN
References and
documentation are available.
Number of amino acids: 316
Molecular weight: 37159.15
Theoretical pI: 9.42
Total number of negatively charged residues (Asp + Glu): 49
Total number of positively charged residues (Arg + Lys): 63
Atomic composition:
Carbon C 1658
Hydrogen H 2676
Nitrogen N 464
Oxygen O 475
Sulfur S 14
Formula: C
1658H
2676N
464O
475S
14
Total number of atoms: 5287
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 34170
Abs 0.1% (=1 g/l) 0.920, assuming all pairs of Cys residues form cystines
Ext. coefficient 33920
Abs 0.1% (=1 g/l) 0.913, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 54.24
This classifies the protein as unstable.
Aliphatic index: 78.35
Grand average of hydropathicity (GRAVY): -0.763