ProtParam
User-provided sequence:
10 20 30 40 50 60
MVSVTRSVFG DLPSGAGTVE KFQLQSDQLR VDIISWGCTI TALEVKDRQG RASDVVLGFA
70 80 90 100 110 120
ELKEYLQKHP YFGAVVGRVA NRIAKGTFTL DGKEYKLAIN NGPNSLHGGV RGFDKVLWTP
130 140 150 160 170 180
RVLSNGIEFS RVSPDGEEGY PGELKVWVTY TLDGGELVVN YRAQASQTTP VNLTNHSYFN
190 200 210 220 230 240
LAGQGSPNIY DHEVTIEADA FLPVDETLIP TGEIAPVQGT AFDLRKPVEL GKHLQEFHIN
250 260 270 280 290 300
GFDHNFCLKR SKEKQFCARV HHAGSGRVLE VYTTQPGIQF YTGNFLDGTL KGKTGAVYPK
310 320 330 340
HSGFCLETQN WPNAVNQPHF PPVLLKPGEE YNHTTWFVFS VA
References and
documentation are available.
Number of amino acids: 342
Molecular weight: 37815.64
Theoretical pI: 6.31
Total number of negatively charged residues (Asp + Glu): 36
Total number of positively charged residues (Arg + Lys): 32
Atomic composition:
Carbon C 1710
Hydrogen H 2611
Nitrogen N 463
Oxygen O 500
Sulfur S 5
Formula: C
1710H
2611N
463O
500S
5
Total number of atoms: 5289
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 45630
Abs 0.1% (=1 g/l) 1.207, assuming all pairs of Cys residues form cystines
Ext. coefficient 45380
Abs 0.1% (=1 g/l) 1.200, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 27.45
This classifies the protein as stable.
Aliphatic index: 79.74
Grand average of hydropathicity (GRAVY): -0.298