ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVTFNCEVCN DTVPKKNTEK HYYRCPNAYY TCIDCSKTFE DGVSYKNHTS CISEDEKYQK 

        70         80         90        100        110        120 
ALYKGNKKQK QKQQQKQQQK QHQHQPVATP AKKVEKPVIK KAEKVEKTSN GIELHKGKSL 

       130        140        150 
YKILKTMKDK GAKKTFLKSL VVDSEGQIRY AKE 

References and documentation are available.

Number of amino acids: 153

Molecular weight: 17738.41

Theoretical pI: 9.54

Amino acid composition: 
Ala (A)   7	  4.6%
Arg (R)   2	  1.3%
Asn (N)   7	  4.6%
Asp (D)   6	  3.9%
Cys (C)   6	  3.9%
Gln (Q)  13	  8.5%
Glu (E)  11	  7.2%
Gly (G)   6	  3.9%
His (H)   5	  3.3%
Ile (I)   6	  3.9%
Leu (L)   6	  3.9%
Lys (K)  31	 20.3%
Met (M)   2	  1.3%
Phe (F)   3	  2.0%
Pro (P)   5	  3.3%
Ser (S)   8	  5.2%
Thr (T)  10	  6.5%
Trp (W)   0	  0.0%
Tyr (Y)   9	  5.9%
Val (V)  10	  6.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 17
Total number of positively charged residues (Arg + Lys): 33

Atomic composition:

Carbon      C	       780
Hydrogen    H	      1262
Nitrogen    N	       220
Oxygen      O	       235
Sulfur      S	         8

Formula: C780H1262N220O235S8
Total number of atoms: 2505

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    13785
Abs 0.1% (=1 g/l)   0.777, assuming all pairs of Cys residues form cystines


Ext. coefficient    13410
Abs 0.1% (=1 g/l)   0.756, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.47
This classifies the protein as unstable.



Aliphatic index: 54.12

Grand average of hydropathicity (GRAVY): -1.172