ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVTGEENVYL KSSLSILKEL SLDELLNVQY DVTTLIAKRV QALQNRNKCV LEEPNSKLAE 

        70         80         90        100        110        120 
ILCHEKNAPQ QSSQTSAGPG EQDSEDFILT QFDEDIKKES AEVHYRNENK HTVQLPLVTM 

       130        140        150        160        170        180 
PPNRHKRKIS EFSSPLNGLN NLSDLEDCSD TVIHEKDNDK ENKTRKLLGI ELENPESTSP 

       190        200        210        220        230        240 
NLYKNVKDNF LFDFNTNPLT KRAWILEDFR PNEDIAPVKR GRRKLERFYA QVGKPEDSKH 

       250        260        270        280        290        300 
RSLSVVIESQ NSDYEFAFDN LRNRSKSPPG FGRLDFPSTQ EGNEDKKKSQ EIIRRKTKYR 

       310        320        330        340 
FLMASNNKIP PYEREYVFKR EQLNQIVDDG CFFWSDKLLQ IYARC 

References and documentation are available.

Number of amino acids: 345

Molecular weight: 40097.02

Theoretical pI: 6.17

Amino acid composition: 
Ala (A)  12	  3.5%
Arg (R)  22	  6.4%
Asn (N)  28	  8.1%
Asp (D)  23	  6.7%
Cys (C)   5	  1.4%
Gln (Q)  16	  4.6%
Glu (E)  33	  9.6%
Gly (G)  11	  3.2%
His (H)   6	  1.7%
Ile (I)  16	  4.6%
Leu (L)  34	  9.9%
Lys (K)  31	  9.0%
Met (M)   3	  0.9%
Phe (F)  16	  4.6%
Pro (P)  18	  5.2%
Ser (S)  28	  8.1%
Thr (T)  14	  4.1%
Trp (W)   2	  0.6%
Tyr (Y)  10	  2.9%
Val (V)  17	  4.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 56
Total number of positively charged residues (Arg + Lys): 53

Atomic composition:

Carbon      C	      1762
Hydrogen    H	      2788
Nitrogen    N	       500
Oxygen      O	       554
Sulfur      S	         8

Formula: C1762H2788N500O554S8
Total number of atoms: 5612

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    26150
Abs 0.1% (=1 g/l)   0.652, assuming all pairs of Cys residues form cystines


Ext. coefficient    25900
Abs 0.1% (=1 g/l)   0.646, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 57.86
This classifies the protein as unstable.



Aliphatic index: 74.29

Grand average of hydropathicity (GRAVY): -0.905