ProtParam
User-provided sequence:
10 20 30 40 50 60
MVTILSTPLS PRLTFLCETK LSLSRSNRSV CCSLSEEPKD QCLSRRSLVY VLVASPCLLL
70 80 90 100 110 120
PALSSSAKTK SKSPYDERRL LEQNKRIQRE NNAPDEFPNF VREGFEVKVL ASDNYIKADS
130 140 150 160 170 180
GLIYRDFNVG QGDFPKDGQQ VTFHYIGYNE SGRRIDSTYI QGSPARIRMG TNALVPGFEM
190 200 210 220 230 240
GIRDMKPGGR RRIIIPPELG PPVGPSTFFS SKQFEVFDVE LLSIQNCERR TIIGFYSDVT
CS
References and
documentation are available.
Number of amino acids: 242
Molecular weight: 27192.06
Theoretical pI: 8.83
Total number of negatively charged residues (Asp + Glu): 27
Total number of positively charged residues (Arg + Lys): 32
Atomic composition:
Carbon C 1199
Hydrogen H 1911
Nitrogen N 337
Oxygen O 362
Sulfur S 11
Formula: C
1199H
1911N
337O
362S
11
Total number of atoms: 3820
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 12295
Abs 0.1% (=1 g/l) 0.452, assuming all pairs of Cys residues form cystines
Ext. coefficient 11920
Abs 0.1% (=1 g/l) 0.438, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 60.00
This classifies the protein as unstable.
Aliphatic index: 80.91
Grand average of hydropathicity (GRAVY): -0.351