ProtParam
User-provided sequence:
10 20 30 40 50 60
MVTKAEKQFA KNFNIDDLPD VPAGLPPHLK AQQTRVVSKN NAPAHTASAI YSGTYVSSTE
70 80 90 100 110 120
EDDNVKLGNF YDNFKVDVVS LTKTDMEFDM IGIDAAFANA FRRILIAEVP SMAIEKVLIA
130 140 150 160 170 180
YNTSVIIDEV LAHRMGLIPI AADPRLFEYL SEHDQANEKN TIVFKLHVKC PKNRPRLKVL
190 200 210 220 230 240
TSDLKWLPNG SELLRESENK TSKPKTYTSF SCSQDSLPEF ANNPITPCDL DILIAKLAPG
250 260 270 280 290 300
QEIELEAHAV KGIGKTHAKW SPVGTAWYRM HPEVRSIYIN LSYALWETAL CFVFPHPNIY
VS
References and
documentation are available.
Number of amino acids: 302
Molecular weight: 33806.73
Theoretical pI: 6.15
Total number of negatively charged residues (Asp + Glu): 36
Total number of positively charged residues (Arg + Lys): 32
Atomic composition:
Carbon C 1526
Hydrogen H 2384
Nitrogen N 400
Oxygen O 447
Sulfur S 10
Formula: C
1526H
2384N
400O
447S
10
Total number of atoms: 4767
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 37150
Abs 0.1% (=1 g/l) 1.099, assuming all pairs of Cys residues form cystines
Ext. coefficient 36900
Abs 0.1% (=1 g/l) 1.091, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 39.05
This classifies the protein as stable.
Aliphatic index: 90.13
Grand average of hydropathicity (GRAVY): -0.203