ProtParam
User-provided sequence:
10 20 30 40 50 60
MVTKHQIEED HLDGATTDPE VKRVKLENNV EEIQPEQAET NKQEGTDKEN KGKFEKETER
70 80 90 100 110 120
IGGSEVVTDV EKGIVKFEFD GVEYTFKERP SVVEENEGKI EFRVVNNDNT KENMMVLTGL
130 140 150 160 170 180
KNIFQKQLPK MPKEYIARLV YDRSHLSMAV IRKPLTVVGG ITYRPFDKRE FAEIVFCAIS
190 200 210 220 230 240
STEQVRGYGA HLMNHLKDYV RNTSNIKYFL TYADNYAIGY FKKQGFTKEI TLDKSIWMGY
250 260 270 280 290 300
IKDYEGGTLM QCSMLPRIRY LDAGKILLLQ EAALRRKIRT ISKSHIVRPG LEQFKDLNNI
310 320 330 340 350 360
KPIDPMTIPG LKEAGWTPEM DALAQRPKRG PHDAAIQNIL TELQNHAAAW PFLQPVNKEE
370 380 390 400 410 420
VPDYYDFIKE PMDLSTMEIK LESNKYQKME DFIYDARLVF NNCRMYNGEN TSYYKYANRL
430
EKFFNNKVKE IPEYSHLID
References and
documentation are available.
Number of amino acids: 439
Molecular weight: 51069.34
Theoretical pI: 6.22
Total number of negatively charged residues (Asp + Glu): 68
Total number of positively charged residues (Arg + Lys): 64
Atomic composition:
Carbon C 2285
Hydrogen H 3581
Nitrogen N 611
Oxygen O 680
Sulfur S 18
Formula: C
2285H
3581N
611O
680S
18
Total number of atoms: 7175
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 49405
Abs 0.1% (=1 g/l) 0.967, assuming all pairs of Cys residues form cystines
Ext. coefficient 49280
Abs 0.1% (=1 g/l) 0.965, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 42.82
This classifies the protein as unstable.
Aliphatic index: 77.27
Grand average of hydropathicity (GRAVY): -0.653