ProtParam
User-provided sequence:
10 20 30 40 50 60
MVTQTNPVPV TYPTDAYIPT YLPDDKVSNL ADLKKLIEMD SRLDLYLTRR RLDTSINLPT
70 80 90 100 110 120
NTKTKDHPPN KEMLRIYVYN TTESSPRSDS GTPADSGKTT WTLRIEGKLL HESANGKHPF
130 140 150 160 170 180
SEFLEGVAVD FKRLKPLGMG KKRKRDSSLS LPLNLQQPEY NDQDSTMGDN DNGEDEDSAE
190 200 210 220 230 240
AESREEIVDA LEWNYDENNV VEFDGIDIKR QGKDNLRCSI TIQLRGVDGG KVQYSPNLAT
250 260 270 280 290 300
LIGMQTGSVN DAVYSIYKYI LINNLFVTEQ TEAQDGSNDA EDSSNENNNK NGAGDDDGVE
310 320 330 340 350 360
GSTPKDKPEL GEVKLDSLLQ KVLDTNAAHL PLMNVVQTVN KLVSPLPPII LDYTIDLSKD
370 380 390 400 410 420
TTYGATTLDV DVSHILHQPQ PQPNLQKEEE TDAEDTAKLR EITKLALQLN SSAQKYQFFH
430 440 450 460 470 480
ELSLHPRETL THYLWSSKQN ELVLQGDQYF NEDAARTSDI YSNNNNDRSL MGNISLLYSQ
GRL
References and
documentation are available.
Number of amino acids: 483
Molecular weight: 54164.98
Theoretical pI: 4.69
Total number of negatively charged residues (Asp + Glu): 78
Total number of positively charged residues (Arg + Lys): 49
Atomic composition:
Carbon C 2357
Hydrogen H 3731
Nitrogen N 651
Oxygen O 793
Sulfur S 9
Formula: C
2357H
3731N
651O
793S
9
Total number of atoms: 7541
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 44810
Abs 0.1% (=1 g/l) 0.827, assuming all pairs of Cys residues form cystines
Ext. coefficient 44810
Abs 0.1% (=1 g/l) 0.827, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 43.42
This classifies the protein as unstable.
Aliphatic index: 80.93
Grand average of hydropathicity (GRAVY): -0.744