ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVVGTKKYSN LDFVPTISDS EDDVPILDSS DDEKVEAKKT TKKRKGKNNK KKVSEGDNLD 

        70         80         90        100        110        120 
EDVHEDLDAG FKFDLDADDT TSNFQGWNFL AEGESNKDDA EAFVKKDVDL DKIIRRKGGL 

       130        140        150        160        170        180 
VKMAHIDSKQ EEETEKEKVE KENDSDDEEL AMDGFGMGAP MNNGDENQSE EEEEEEEKEE 

       190        200        210        220        230        240 
EEEEEEEQEE MTLEKGGKDD EIDEEDDSEE AKADFYAPET EGDEAKKQMY ENFNSLSLSR 

       250        260        270        280        290        300 
PVLKGLASLG YVKPSPIQSA TIPIALLGKD IIAGAVTGSG KTAAFMIPII ERLLYKPAKI 

       310        320        330        340        350        360 
ASTRVIVLLP TRELAIQVAD VGKQIARFVS GITFGLAVGG LNLRQQEQML KSRPDIVIAT 

       370        380        390        400        410        420 
PGRFIDHIRN SASFNVDSVE ILVMDEADRM LEEGFQDELN EIMGLLPSNR QNLLFSATMN 

       430        440        450        460        470        480 
SKIKSLVSLS LKKPVRIMID PPKKAATKLT QEFVRIRKRD HLKPALLFNL IRKLDPTGQK 

       490        500        510        520        530        540 
RIVVFVARKE TAHRLRIIMG LLGMSVGELH GSLTQEQRLD SVNKFKNLEV PVLICTDLAS 

       550        560        570        580        590        600 
RGLDIPKIEV VINYDMPKSY EIYLHRVGRT ARAGREGRSV TFVGESSQDR SIVRAAIKSV 

       610        620        630        640        650        660 
EENKSLTQGK ALGRNVDWVQ IEETNKLVES MNDTIEDILV EEKEEKEILR AEMQLRKGEN 

       670        680        690        700        710        720 
MLKHKKEIQA RPRRTWFQSE SDKKNSKVLG ALSRNKKVTN SKKRKREEAK ADGNGARSYR 

       730        740        750 
KTKTDRIADQ ERTFKKQKST NSNKKKGFKS RR 

References and documentation are available.

Number of amino acids: 752

Molecular weight: 84843.11

Theoretical pI: 5.59

Amino acid composition: 
Ala (A)  48	  6.4%
Arg (R)  47	  6.2%
Asn (N)  35	  4.7%
Asp (D)  57	  7.6%
Cys (C)   1	  0.1%
Gln (Q)  25	  3.3%
Glu (E)  84	 11.2%
Gly (G)  46	  6.1%
His (H)   8	  1.1%
Ile (I)  46	  6.1%
Leu (L)  62	  8.2%
Lys (K)  81	 10.8%
Met (M)  20	  2.7%
Phe (F)  24	  3.2%
Pro (P)  23	  3.1%
Ser (S)  52	  6.9%
Thr (T)  34	  4.5%
Trp (W)   3	  0.4%
Tyr (Y)   9	  1.2%
Val (V)  47	  6.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 141
Total number of positively charged residues (Arg + Lys): 128

Atomic composition:

Carbon      C	      3688
Hydrogen    H	      6037
Nitrogen    N	      1053
Oxygen      O	      1190
Sulfur      S	        21

Formula: C3688H6037N1053O1190S21
Total number of atoms: 11989

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    29910
Abs 0.1% (=1 g/l)   0.353, assuming all pairs of Cys residues form cystines


Ext. coefficient    29910
Abs 0.1% (=1 g/l)   0.353, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.93
This classifies the protein as unstable.



Aliphatic index: 80.52

Grand average of hydropathicity (GRAVY): -0.741