ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVVINGVKYA CETCIRGHRA AQCTHTDGPL QMIRRKGRPS TTCGHCKELR RTKNFNPSGG 

        70         80         90        100        110        120 
CMCASARRPA VGSKEDETRC RCDEGEPCKC HTKRKSSRKS KGGSCHRRAN DEAAHVNGLG 

       130        140        150        160        170        180 
IADLDVLLGL NGRSSDVDMT TTLPSLKPPL QNGEIKADSI DNLDLASLDP LEQSPSISME 

       190        200        210        220 
PVSINETGSA YTTTNTALND IDIPFSINEL NELYKQVSSH NSHSQ 

References and documentation are available.

Number of amino acids: 225

Molecular weight: 24425.42

Theoretical pI: 7.97

Amino acid composition: 
Ala (A)  14	  6.2%
Arg (R)  16	  7.1%
Asn (N)  14	  6.2%
Asp (D)  14	  6.2%
Cys (C)  12	  5.3%
Gln (Q)   6	  2.7%
Glu (E)  13	  5.8%
Gly (G)  17	  7.6%
His (H)   8	  3.6%
Ile (I)  11	  4.9%
Leu (L)  17	  7.6%
Lys (K)  13	  5.8%
Met (M)   5	  2.2%
Phe (F)   2	  0.9%
Pro (P)  12	  5.3%
Ser (S)  23	 10.2%
Thr (T)  16	  7.1%
Trp (W)   0	  0.0%
Tyr (Y)   3	  1.3%
Val (V)   9	  4.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 27
Total number of positively charged residues (Arg + Lys): 29

Atomic composition:

Carbon      C	      1017
Hydrogen    H	      1670
Nitrogen    N	       322
Oxygen      O	       342
Sulfur      S	        17

Formula: C1017H1670N322O342S17
Total number of atoms: 3368

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     5220
Abs 0.1% (=1 g/l)   0.214, assuming all pairs of Cys residues form cystines


Ext. coefficient     4470
Abs 0.1% (=1 g/l)   0.183, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 51.57
This classifies the protein as unstable.



Aliphatic index: 66.36

Grand average of hydropathicity (GRAVY): -0.667