ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVVKKRKLAT EAGGSDERPK YLPGKHPKNQ EKTPHVDYNA PLNPKSELFL DDWHIPKFNR 

        70         80         90        100        110        120 
FISFTLDVLI DKYKDIFKDF IKLPSRKFHP QYYYKIQQPM SINEIKSRDY EYEDGPSNFL 

       130        140        150        160        170        180 
LDVELLTKNC QAYNEYDSLI VKNSMQVVML IEFEVLKAKN LKRNYLINSE VKAKLLHYLN 

       190        200        210        220        230        240 
KLVDATEKKI NQALLGASSP KNLDDKVKLS EPFMELVDKD ELPEYYEIVH SPMALSIVKQ 

       250        260        270        280        290        300 
NLEIGQYSKI YDFIIDMLLV FQNAHIFNDP SALIYKDATT LTNYFNYLIQ KEFFPELQDL 

       310        320        330        340        350        360 
NERGEINLEF DKFEFENYLA IGGGGPAAAG ALAISALDND IEPESNREDL IDQADYDFNH 

       370        380        390        400        410        420 
FEGLGNGYNR SLLTEDYLLN PNNFKKLIAI PETVQSEVKN ERSTTSDIEK TNSLESEHLK 

       430        440        450        460        470        480 
IPKYNVIKSM QKEMQSLSEQ HTMEYKPYKL IQQIYIFSSK NLYSQATKPL LGSRPSCNQN 

       490        500        510        520        530        540 
WVEYIFNGNE LSQNENAFSF MLQPMQTFLT LQSHLTSSLK DTETLLTINK EPVKSRTSNV 

       550        560        570        580        590        600 
NSNLSQPQQQ ENDVIGNDTK QDIENLTIGG GNNNDIVGND NDKRNNITEI FDIRLSEGLN 

       610        620 
HLMFRCEDKI SHETEFMNFW INVLP 

References and documentation are available.

Number of amino acids: 625

Molecular weight: 72275.68

Theoretical pI: 5.11

Amino acid composition: 
Ala (A)  24	  3.8%
Arg (R)  15	  2.4%
Asn (N)  58	  9.3%
Asp (D)  40	  6.4%
Cys (C)   3	  0.5%
Gln (Q)  30	  4.8%
Glu (E)  52	  8.3%
Gly (G)  23	  3.7%
His (H)  13	  2.1%
Ile (I)  45	  7.2%
Leu (L)  68	 10.9%
Lys (K)  53	  8.5%
Met (M)  14	  2.2%
Phe (F)  31	  5.0%
Pro (P)  29	  4.6%
Ser (S)  44	  7.0%
Thr (T)  26	  4.2%
Trp (W)   3	  0.5%
Tyr (Y)  29	  4.6%
Val (V)  25	  4.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 92
Total number of positively charged residues (Arg + Lys): 68

Atomic composition:

Carbon      C	      3242
Hydrogen    H	      5034
Nitrogen    N	       840
Oxygen      O	       997
Sulfur      S	        17

Formula: C3242H5034N840O997S17
Total number of atoms: 10130

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    59835
Abs 0.1% (=1 g/l)   0.828, assuming all pairs of Cys residues form cystines


Ext. coefficient    59710
Abs 0.1% (=1 g/l)   0.826, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.25
This classifies the protein as stable.



Aliphatic index: 85.95

Grand average of hydropathicity (GRAVY): -0.584