ProtParam
User-provided sequence:
10 20 30 40 50 60
MVVLDKKLLE RLTSRKVPLE ELEDMEKRCF LSTFTYQDAF DLGTYIRNAV KENFPEKPVA
70 80 90 100 110 120
IDISLPNGHC LFRTVTYGGS ALDNDFWIQR KKKTALRFGH SSFYMGCKKS DKTPEEKFFV
130 140 150 160 170
DSKEYAFHGG AVLIQSERSD YPYACLTISG LKQEEDHLMA VSSLIAFANE SLEEDLNLD
References and
documentation are available.
Number of amino acids: 179
Molecular weight: 20484.30
Theoretical pI: 5.30
Total number of negatively charged residues (Asp + Glu): 29
Total number of positively charged residues (Arg + Lys): 23
Atomic composition:
Carbon C 918
Hydrogen H 1423
Nitrogen N 237
Oxygen O 278
Sulfur S 8
Formula: C
918H
1423N
237O
278S
8
Total number of atoms: 2864
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 16180
Abs 0.1% (=1 g/l) 0.790, assuming all pairs of Cys residues form cystines
Ext. coefficient 15930
Abs 0.1% (=1 g/l) 0.778, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 52.42
This classifies the protein as unstable.
Aliphatic index: 79.55
Grand average of hydropathicity (GRAVY): -0.381