ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVVNNPNNWH WVDKNCIGWA KEYFKQKLVG VEAGSVKDKK YAKIKSVSSI EGDCEVNQRK 

        70         80         90        100        110        120 
GKVISLFDLK ITVLIEGHVD SKDGSALPFE GSINVPEVAF DSEASSYQFD ISIFKETSEL 

       130        140        150        160        170        180 
SEAKPLIRSE LLPKLRQIFQ QFGKDLLATH GNDIQVPESQ VKSNYTRGNQ KSSFTEIKDS 

       190        200        210        220        230        240 
ASKPKKNALP SSTSTSAPVS STNKVPQNGS GNSTSIYLEP TFNVPSSELY ETFLDKQRIL 

       250        260        270        280        290        300 
AWTRSAQFFN SGPKLETKEK FELFGGNVIS ELVSCEKDKK LVFHWKLKDW SAPFNSTIEM 

       310        320        330        340        350 
TFHESQEFHE TKLQVKWTGI PVGEEDRVRA NFEEYYVRSI KLTFGFGAVL 

References and documentation are available.

Number of amino acids: 350

Molecular weight: 39435.53

Theoretical pI: 7.02

Amino acid composition: 
Ala (A)  16	  4.6%
Arg (R)   9	  2.6%
Asn (N)  19	  5.4%
Asp (D)  15	  4.3%
Cys (C)   3	  0.9%
Gln (Q)  14	  4.0%
Glu (E)  30	  8.6%
Gly (G)  20	  5.7%
His (H)   6	  1.7%
Ile (I)  19	  5.4%
Leu (L)  25	  7.1%
Lys (K)  36	 10.3%
Met (M)   2	  0.6%
Phe (F)  22	  6.3%
Pro (P)  15	  4.3%
Ser (S)  40	 11.4%
Thr (T)  18	  5.1%
Trp (W)   7	  2.0%
Tyr (Y)   8	  2.3%
Val (V)  26	  7.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 45
Total number of positively charged residues (Arg + Lys): 45

Atomic composition:

Carbon      C	      1777
Hydrogen    H	      2757
Nitrogen    N	       465
Oxygen      O	       540
Sulfur      S	         5

Formula: C1777H2757N465O540S5
Total number of atoms: 5544

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    50545
Abs 0.1% (=1 g/l)   1.282, assuming all pairs of Cys residues form cystines


Ext. coefficient    50420
Abs 0.1% (=1 g/l)   1.279, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 36.43
This classifies the protein as stable.



Aliphatic index: 75.14

Grand average of hydropathicity (GRAVY): -0.500