ProtParam
User-provided sequence:
10 20 30 40 50 60
MVVNTIYIAR HGYRSNWLPE GPYPDPLTGI DSDVPLAEHG VQQAKELAHY LLSLDNQPEA
70 80 90 100 110 120
AFASPFYRCL ETVQPIAKLL EIPVYLERGI GEWYRPDRKP VIPVPAGYEI LSKFFPGVIS
130 140 150 160 170 180
QEWDSTLTPN EKGETEQEMY MRFKKFWPLF IERVEKEYPN VECILLVTHA ASKIALGMSL
190 200 210 220 230 240
LGYDNPRMSL NENGDKIRSG SCSLDKYEIL KKSYDTIDET DDQTSFTYIP FSDRKWVLTM
250 260 270 280 290 300
NGNTEFLSSG EEMNWNFDCV AEAGSDADIK KRQMTKKTSS PIPEADDQTE VETVYISVDI
310 320 330 340 350 360
PSGNYKERTE IAKSAILQYS GLETDAPLFR IGNRLYEGSW ERLVGTELAF PNAAHVHKKT
370 380 390 400 410 420
AGLLSPTEEN ETTNAGQSKG SSTANDPNIQ IQEEDVGLPD STNTSRDHTG DKEEVQSEKI
430
YRIKERIVLS NVRPM
References and
documentation are available.
Number of amino acids: 435
Molecular weight: 49146.10
Theoretical pI: 4.83
Total number of negatively charged residues (Asp + Glu): 69
Total number of positively charged residues (Arg + Lys): 46
Atomic composition:
Carbon C 2181
Hydrogen H 3385
Nitrogen N 577
Oxygen O 690
Sulfur S 13
Formula: C
2181H
3385N
577O
690S
13
Total number of atoms: 6846
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 67060
Abs 0.1% (=1 g/l) 1.365, assuming all pairs of Cys residues form cystines
Ext. coefficient 66810
Abs 0.1% (=1 g/l) 1.359, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 39.78
This classifies the protein as stable.
Aliphatic index: 76.67
Grand average of hydropathicity (GRAVY): -0.576