ProtParam
User-provided sequence:
10 20 30 40 50 60
MVYEATPFDP ITVKPSDKRR VAYFYDADVG NYAYGAGHPM KPHRIRMAHS LIMNYGLYKK
70 80 90 100 110 120
MEIYRAKPAT KQEMCQFHTD EYIDFLSRVT PDNLEMFKRE SVKFNVGDDC PVFDGLYEYC
130 140 150 160 170 180
SISGGGSMEG AARLNRGKCD VAVNYAGGLH HAKKSEASGF CYLNDIVLGI IELLRYHPRV
190 200 210 220 230 240
LYIDIDVHHG DGVEEAFYTT DRVMTCSFHK YGEFFPGTGE LRDIGVGAGK NYAVNVPLRD
250 260 270 280 290 300
GIDDATYRSV FEPVIKKIME WYQPSAVVLQ CGGDSLSGDR LGCFNLSMEG HANCVNYVKS
310 320 330 340 350 360
FGIPMMVVGG GGYTMRNVAR TWCFETGLLN NVVLDKDLPY NEYYEYYGPD YKLSVRPSNM
370 380 390 400 410 420
FNVNTPEYLD KVMTNIFANL ENTKYAPSVQ LNHTPRDAED LGDVEEDSAE AKDTKGGSQY
430
ARDLHVEHDN EFY
References and
documentation are available.
Number of amino acids: 433
Molecular weight: 48904.08
Theoretical pI: 5.29
Total number of negatively charged residues (Asp + Glu): 61
Total number of positively charged residues (Arg + Lys): 44
Atomic composition:
Carbon C 2176
Hydrogen H 3287
Nitrogen N 579
Oxygen O 657
Sulfur S 26
Formula: C
2176H
3287N
579O
657S
26
Total number of atoms: 6725
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 59305
Abs 0.1% (=1 g/l) 1.213, assuming all pairs of Cys residues form cystines
Ext. coefficient 58680
Abs 0.1% (=1 g/l) 1.200, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 27.77
This classifies the protein as stable.
Aliphatic index: 70.21
Grand average of hydropathicity (GRAVY): -0.418