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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVYEATPFDP ITVKPSDKRR VAYFYDADVG NYAYGAGHPM KPHRIRMAHS LIMNYGLYKK

70 80 90 100 110 120
MEIYRAKPAT KQEMCQFHTD EYIDFLSRVT PDNLEMFKRE SVKFNVGDDC PVFDGLYEYC

130 140 150 160 170 180
SISGGGSMEG AARLNRGKCD VAVNYAGGLH HAKKSEASGF CYLNDIVLGI IELLRYHPRV

190 200 210 220 230 240
LYIDIDVHHG DGVEEAFYTT DRVMTCSFHK YGEFFPGTGE LRDIGVGAGK NYAVNVPLRD

250 260 270 280 290 300
GIDDATYRSV FEPVIKKIME WYQPSAVVLQ CGGDSLSGDR LGCFNLSMEG HANCVNYVKS

310 320 330 340 350 360
FGIPMMVVGG GGYTMRNVAR TWCFETGLLN NVVLDKDLPY NEYYEYYGPD YKLSVRPSNM

370 380 390 400 410 420
FNVNTPEYLD KVMTNIFANL ENTKYAPSVQ LNHTPRDAED LGDVEEDSAE AKDTKGGSQY

430
ARDLHVEHDN EFY


References and documentation are available.

Number of amino acids: 433 Molecular weight: 48904.08 Theoretical pI: 5.29
Amino acid composition: 
Ala (A) 27 6.2% Arg (R) 21 4.8% Asn (N) 24 5.5% Asp (D) 33 7.6% Cys (C) 10 2.3% Gln (Q) 6 1.4% Glu (E) 28 6.5% Gly (G) 39 9.0% His (H) 14 3.2% Ile (I) 17 3.9% Leu (L) 28 6.5% Lys (K) 23 5.3% Met (M) 16 3.7% Phe (F) 19 4.4% Pro (P) 20 4.6% Ser (S) 21 4.8% Thr (T) 18 4.2% Trp (W) 2 0.5% Tyr (Y) 32 7.4% Val (V) 35 8.1% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 61 Total number of positively charged residues (Arg + Lys): 44 Atomic composition: Carbon C 2176 Hydrogen H 3287 Nitrogen N 579 Oxygen O 657 Sulfur S 26 Formula: C2176H3287N579O657S26 Total number of atoms: 6725 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 59305 Abs 0.1% (=1 g/l) 1.213, assuming all pairs of Cys residues form cystines Ext. coefficient 58680 Abs 0.1% (=1 g/l) 1.200, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 27.77 This classifies the protein as stable. Aliphatic index: 70.21 Grand average of hydropathicity (GRAVY): -0.418