ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MWGGGKMAVV SLSPHTAKMR KLFGQASTTM AYDGLKREAE RRTRSDHNIT MVAKDDELYL 

        70         80         90        100        110        120 
YHLTLKKQTN FVHSCIGHFV DLEAGSKREQ SQLCVATETH LELYDTADGE LKLIAKFQNL 

       130        140        150        160        170        180 
FATITSMKSL DLPHSGSRAK ASNWPTFLAL TSDSGNLSIV QIIMHAGALR LKTLVNQPLT 

       190        200        210        220        230        240 
RTTLRRVSPI SYMEIDPNGR CIILSSVEQN KLCFLVDYAQ KLRISSPLEI IRPHMVTLDM 

       250        260        270        280        290        300 
AVVDVNFNNP CFVTLEIDNA ATQLSVHLIF YVLELGLNHI VKKADYLVNP SANFVLSLPD 

       310        320        330        340        350        360 
LSRYNITTSL SDNNYDADYD TLFNPFVVIG FENHILVKDM NGFFSLKVEI PKRSITNSRH 

       370        380        390        400        410        420 
KNVTIISGIV QKLKNDFFVL LQSNHGDLFK LTVSPDTNDR NRPLVQLSYF DTIQNSHQLH 

       430        440        450        460        470        480 
IFKNGYLFAL SEMNNNFLFQ FEKLGVEKND FSNVLTSKDP NKSLVFEPSI KLQNLSILSQ 

       490        500        510        520        530        540 
QLNLNPSIKS QIVSDSPLSI ATKHFTNNKI ITLTNAVNYS NLISTSLPPN ATKLWLIPDP 

       550        560        570        580        590        600 
ATTGDNNTLL FITFPKKTMI LQIDNESMEE LTPDEATRSA FKLSQDTTIH TCLMGSHSII 

       610        620        630        640        650        660 
QVCTAELRHI VPTGKSRYSN KLTWVPPAGI RIVCATSSKT QLIISLSNYE LVYFKIDVSS 

       670        680        690        700        710        720 
DSLIELTTHP ELDTMPSKVA IVQDTQHADL LAIADNEGMI KIMSLKDQKE DFLTVISLQL 

       730        740        750        760        770        780 
VSEKISDMIM VRDSSIGQLN LHVGLENGVY MKFHIGDVDG SFTDIKRRFL GLKPVSLSYL 

       790        800        810        820        830        840 
REISVSLNNE EEEEEEEDDD DEKEEEEINS SGAKWMSCVV CHSSSTWVSY TWKNVWTIRQ 

       850        860        870        880        890        900 
LKDQNMLSCS KFVNADVAIN GVCSISSSGR LNIGRVSNFP TLDNWFHVHE SSVNKQENGG 

       910        920        930        940        950        960 
GDESNEEEED EMEEEMEMLQ ISTFRPRTIL SFPNNPKSIL FIDNHSGKKQ CRISLQIDGE 

       970        980        990       1000       1010       1020 
CLKFGSSDHL YKILDDIDCV SAAIIDFTRQ ADHLIICAGD KRLLTYKILV NKDKLSFDIE 

      1030       1040       1050       1060       1070       1080 
LLHQTEIISP IHAMLKFKNF LLTAMGSTIV LYGLGKKQLL RRSVTQTPVS ITKIVSMHQW 

      1090       1100       1110       1120       1130       1140 
NYERLAVGDI HESVTLFIWD PAGNVFIPYV DDSVKRHVTV LKFLDEATVI GADRYGNAWT 

      1150       1160       1170       1180       1190       1200 
LRSPPECEKI MSNHDPSELS NGAIKYPLDV ITLQQKLPNT YDCKFKFQLL NHFFVNDIIT 

      1210       1220       1230       1240       1250       1260 
DFHILDSLSN SDRPGCIYMG LQGTVGCFIP LLSKGNVFMM GNIENIMAEA DDTFYLDYES 

      1270       1280       1290       1300       1310       1320 
RKKNNNMRKE DDEEESGSVV LQGRHGIEDE IICEGSCSIL GRDHQEYRSY YAPVRKVIDG 

      1330       1340       1350       1360 
DLCENFLRLS LNEQEFLAKN LKSVQVEDII QTINEVRTNY M 

References and documentation are available.

Number of amino acids: 1361

Molecular weight: 153784.46

Theoretical pI: 5.52

Amino acid composition: 
Ala (A)  56	  4.1%
Arg (R)  50	  3.7%
Asn (N)  90	  6.6%
Asp (D)  90	  6.6%
Cys (C)  23	  1.7%
Gln (Q)  49	  3.6%
Glu (E)  82	  6.0%
Gly (G)  61	  4.5%
His (H)  40	  2.9%
Ile (I) 107	  7.9%
Leu (L) 147	 10.8%
Lys (K)  83	  6.1%
Met (M)  36	  2.6%
Phe (F)  60	  4.4%
Pro (P)  47	  3.5%
Ser (S) 125	  9.2%
Thr (T)  82	  6.0%
Trp (W)  12	  0.9%
Tyr (Y)  35	  2.6%
Val (V)  86	  6.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 172
Total number of positively charged residues (Arg + Lys): 133

Atomic composition:

Carbon      C	      6831
Hydrogen    H	     10807
Nitrogen    N	      1825
Oxygen      O	      2087
Sulfur      S	        59

Formula: C6831H10807N1825O2087S59
Total number of atoms: 21609

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   119525
Abs 0.1% (=1 g/l)   0.777, assuming all pairs of Cys residues form cystines


Ext. coefficient   118150
Abs 0.1% (=1 g/l)   0.768, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.23
This classifies the protein as unstable.



Aliphatic index: 95.22

Grand average of hydropathicity (GRAVY): -0.208