ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MWINRLVKHP SYSVLRFYTK RLCTVSVKSL REFGVLPNST ICHSVYPRRT YVMGRAVIND 

        70         80         90        100        110        120 
ILIKKSYSTH TVCAIDRSKD ENNGSAYDKF EAKGIPIDVH TLKRIISSSG MDESEFSKSI 

       130        140        150        160        170        180 
SYLFAKTVDP EPKDVLSLED LSFLLSKLYT QRFQIRRICR DINAKYSEFW FKLFSLYAEK 

       190        200        210        220        230        240 
VDAKRNQVNL RNTKLDACEI FDANLMIKNF IELGQLGKAQ KILSFILDRN PDILLSPKNA 

       250        260        270        280        290        300 
DISTIVHFLQ LRCGALAPYW KIPDNSEQKQ GFLRKMVRLG AKNTSIRLSS TYKAMDHQTL 

       310        320        330        340        350        360 
LKIADLALQE KKLLNSEDLL STLIQSFGHL GQTQILERCI EHIWQISPQE FPSHVVIKHR 

       370        380        390        400        410        420 
GCYPSSKILV SILVSFYFND HDLHRGLSIL DSFIKHYPDV KLDALFWRRL FQLSHFAWTP 

       430        440        450        460        470        480 
ANDKKATSVV RCWHLMKQWY ASKRLRPSVD YETLRQLYDI MKKTGNFPLG IDVLRSFKPG 

       490        500        510        520        530        540 
IERTRAENAG KVNNIIIKYQ KCIIKELVNR GRFSAVREFI DSYGFDRKMT KDLNIFCANR 

       550        560        570        580 
MFLRSKKMKN KIENKKEREK VRLDSFDDDE DDGMIIGSLW 

References and documentation are available.

Number of amino acids: 580

Molecular weight: 67523.41

Theoretical pI: 9.57

Amino acid composition: 
Ala (A)  27	  4.7%
Arg (R)  40	  6.9%
Asn (N)  27	  4.7%
Asp (D)  38	  6.6%
Cys (C)  11	  1.9%
Gln (Q)  19	  3.3%
Glu (E)  26	  4.5%
Gly (G)  22	  3.8%
His (H)  15	  2.6%
Ile (I)  48	  8.3%
Leu (L)  62	 10.7%
Lys (K)  54	  9.3%
Met (M)  12	  2.1%
Phe (F)  31	  5.3%
Pro (P)  18	  3.1%
Ser (S)  50	  8.6%
Thr (T)  22	  3.8%
Trp (W)   9	  1.6%
Tyr (Y)  20	  3.4%
Val (V)  29	  5.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 64
Total number of positively charged residues (Arg + Lys): 94

Atomic composition:

Carbon      C	      3048
Hydrogen    H	      4835
Nitrogen    N	       839
Oxygen      O	       847
Sulfur      S	        23

Formula: C3048H4835N839O847S23
Total number of atoms: 9592

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    79925
Abs 0.1% (=1 g/l)   1.184, assuming all pairs of Cys residues form cystines


Ext. coefficient    79300
Abs 0.1% (=1 g/l)   1.174, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.68
This classifies the protein as unstable.



Aliphatic index: 93.12

Grand average of hydropathicity (GRAVY): -0.330