ProtParam
User-provided sequence:
10 20 30 40 50 60
MWKSVCLVLM CVVVTRAQFP RQCMTPEGLR SGTCCPSPTG LANDECGSIS GRGQCVSITA
70 80 90 100 110 120
DRRPHGPQYP HDGRDDRERW PLRFFNRTCQ CNGNFTGFNC GRCRHGLTGP NCDQRITVVR
130 140 150 160 170 180
RNIMQMSADE KRAFVNALDQ AKRTVHPDLV ICTRRFQEIL SPDGASVQVE NITIYNYFVW
190 200 210 220 230 240
THYYSVSKTY MGPGQQSFGG VDFSHEGPGF VTWHRYHLLQ LERDMQDMLG DPSFALPYWN
250 260 270 280 290 300
FAIGGSECDI CTDDLIGARS SFDTNGISSN SVFSQWRVIC ESVEEYETLG TICNNTESSP
310 320 330 340 350 360
IRRNPAGNVA RPMVQRLPEP QDVIDCLELN TFDTPPYYST SSQSFRNTIE GYSAPQGNYD
370 380 390 400 410 420
PVVRSLHNLA HLFLNGTGGQ THLSPNDPIF VLLHTFTDAI FDEWLQRHTA AGTLAYPEEN
430 440 450 460 470 480
APIGHNREFN MVPFWPPIRN AEMFVSAPDN LGYTYEVQWP TRAYTISEII TIAIVAVVLV
490 500 510 520
VAVVGGVIGC AVRARSYRSA EGLEPLLGEQ FRRYSEDERH ASQSVV
References and
documentation are available.
Number of amino acids: 526
Molecular weight: 59033.37
Theoretical pI: 5.69
Total number of negatively charged residues (Asp + Glu): 56
Total number of positively charged residues (Arg + Lys): 44
Atomic composition:
Carbon C 2596
Hydrogen H 3969
Nitrogen N 741
Oxygen O 782
Sulfur S 30
Formula: C
2596H
3969N
741O
782S
30
Total number of atoms: 8118
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 78935
Abs 0.1% (=1 g/l) 1.337, assuming all pairs of Cys residues form cystines
Ext. coefficient 77810
Abs 0.1% (=1 g/l) 1.318, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 52.89
This classifies the protein as unstable.
Aliphatic index: 72.24
Grand average of hydropathicity (GRAVY): -0.326