ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MWLKSLLLCL YSLVLCQVHA APSSGKQITS KDVDLQKKYE PSPPATHRGI ITIEYFDPVS 

        70         80         90        100        110        120 
KSMKEADLTF ELYGTVVPKT VNNFAMLAHG VKAVIEGKDP NDIHTYSYRK TKINKVYPNK 

       130        140        150        160        170        180 
YIQGGVVAPD VGPFTVYGPK FDDENFYLKH DRPERLAMAY FGPDSNTSEF IITTKADGNE 

       190        200        210        220        230        240 
ELDGKSVVFG QITSGLDQLM DAIQYTETDE YGKPQHELRF LYFVLEILKI SNILDLHAAY 

       250        260        270        280        290        300 
TEKVEKFRNG DVSVGSTLEN IFRNDKAYTP LTTSTGTTAY DLNHPISRAL MCLTVLGLCF 

       310 
IAYKGMHEKP HTVSLRHK 

References and documentation are available.

Number of amino acids: 318

Molecular weight: 35779.91

Theoretical pI: 6.42

Amino acid composition: 
Ala (A)  18	  5.7%
Arg (R)   9	  2.8%
Asn (N)  13	  4.1%
Asp (D)  20	  6.3%
Cys (C)   4	  1.3%
Gln (Q)   8	  2.5%
Glu (E)  18	  5.7%
Gly (G)  20	  6.3%
His (H)  11	  3.5%
Ile (I)  18	  5.7%
Leu (L)  30	  9.4%
Lys (K)  26	  8.2%
Met (M)   7	  2.2%
Phe (F)  14	  4.4%
Pro (P)  17	  5.3%
Ser (S)  19	  6.0%
Thr (T)  25	  7.9%
Trp (W)   1	  0.3%
Tyr (Y)  18	  5.7%
Val (V)  22	  6.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 38
Total number of positively charged residues (Arg + Lys): 35

Atomic composition:

Carbon      C	      1618
Hydrogen    H	      2513
Nitrogen    N	       415
Oxygen      O	       478
Sulfur      S	        11

Formula: C1618H2513N415O478S11
Total number of atoms: 5035

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    32570
Abs 0.1% (=1 g/l)   0.910, assuming all pairs of Cys residues form cystines


Ext. coefficient    32320
Abs 0.1% (=1 g/l)   0.903, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 31.36
This classifies the protein as stable.



Aliphatic index: 84.59

Grand average of hydropathicity (GRAVY): -0.294