ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MWLSKFQFPS RSIFKGVFLG HKLPLLVRLT STTTNSKSNG SIPTQYTELS PLLVKQAEKY 

        70         80         90        100        110        120 
EAELKDLDKD LSCGIHFDVN KQKHYAKLSA LTDTFIEYKE KLNELKSLQE MIVSDPSLRA 

       130        140        150        160        170        180 
EAEQEYAELV PQYETTSSRL VNKLLPPHPF ADKPSLLELR PGVGGIEAMI FTQNLLDMYI 

       190        200        210        220        230        240 
GYANYRKWKY RIISKNENES GSGIIDAILS IEEAGSYDRL RFEAGVHRVQ RIPSTETKGR 

       250        260        270        280        290        300 
THTSTAAVVV LPQIGDESAK SIDAYERTFK PGEIRVDIMR ASGKGGQHVN TTDSAVRLTH 

       310        320        330        340        350        360 
IPSGIVVSMQ DERSQHKNKA KAFTILRARL AEKERLEKEE KERKARKSQV SSTNRSDKIR 

       370        380        390        400        410 
TYNFPQNRIT DHRCGFTLLD LPGVLSGERL DEVIEAMSKY DSTERAKELL ESN 

References and documentation are available.

Number of amino acids: 413

Molecular weight: 46770.15

Theoretical pI: 8.85

Amino acid composition: 
Ala (A)  26	  6.3%
Arg (R)  28	  6.8%
Asn (N)  15	  3.6%
Asp (D)  20	  4.8%
Cys (C)   2	  0.5%
Gln (Q)  15	  3.6%
Glu (E)  37	  9.0%
Gly (G)  22	  5.3%
His (H)  10	  2.4%
Ile (I)  25	  6.1%
Leu (L)  41	  9.9%
Lys (K)  34	  8.2%
Met (M)   7	  1.7%
Phe (F)  13	  3.1%
Pro (P)  17	  4.1%
Ser (S)  38	  9.2%
Thr (T)  26	  6.3%
Trp (W)   2	  0.5%
Tyr (Y)  14	  3.4%
Val (V)  21	  5.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 57
Total number of positively charged residues (Arg + Lys): 62

Atomic composition:

Carbon      C	      2064
Hydrogen    H	      3323
Nitrogen    N	       583
Oxygen      O	       636
Sulfur      S	         9

Formula: C2064H3323N583O636S9
Total number of atoms: 6615

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    31985
Abs 0.1% (=1 g/l)   0.684, assuming all pairs of Cys residues form cystines


Ext. coefficient    31860
Abs 0.1% (=1 g/l)   0.681, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.86
This classifies the protein as unstable.



Aliphatic index: 83.37

Grand average of hydropathicity (GRAVY): -0.585