ProtParam
User-provided sequence:
10 20 30 40 50 60
MWLSKFQFPS RSIFKGVFLG HKLPLLVRLT STTTNSKSNG SIPTQYTELS PLLVKQAEKY
70 80 90 100 110 120
EAELKDLDKD LSCGIHFDVN KQKHYAKLSA LTDTFIEYKE KLNELKSLQE MIVSDPSLRA
130 140 150 160 170 180
EAEQEYAELV PQYETTSSRL VNKLLPPHPF ADKPSLLELR PGVGGIEAMI FTQNLLDMYI
190 200 210 220 230 240
GYANYRKWKY RIISKNENES GSGIIDAILS IEEAGSYDRL RFEAGVHRVQ RIPSTETKGR
250 260 270 280 290 300
THTSTAAVVV LPQIGDESAK SIDAYERTFK PGEIRVDIMR ASGKGGQHVN TTDSAVRLTH
310 320 330 340 350 360
IPSGIVVSMQ DERSQHKNKA KAFTILRARL AEKERLEKEE KERKARKSQV SSTNRSDKIR
370 380 390 400 410
TYNFPQNRIT DHRCGFTLLD LPGVLSGERL DEVIEAMSKY DSTERAKELL ESN
References and
documentation are available.
Number of amino acids: 413
Molecular weight: 46770.15
Theoretical pI: 8.85
Total number of negatively charged residues (Asp + Glu): 57
Total number of positively charged residues (Arg + Lys): 62
Atomic composition:
Carbon C 2064
Hydrogen H 3323
Nitrogen N 583
Oxygen O 636
Sulfur S 9
Formula: C
2064H
3323N
583O
636S
9
Total number of atoms: 6615
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 31985
Abs 0.1% (=1 g/l) 0.684, assuming all pairs of Cys residues form cystines
Ext. coefficient 31860
Abs 0.1% (=1 g/l) 0.681, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 41.86
This classifies the protein as unstable.
Aliphatic index: 83.37
Grand average of hydropathicity (GRAVY): -0.585