ProtParam
User-provided sequence:
10 20 30 40 50 60
MWNPILLDTS SFSFQKHVSG VFLQVRNATK RAAGSRTSMK DSAGRRLGPK KYEGQDVSTG
70 80 90 100 110 120
EIIMRQRGTK FYPGENVGIG KDHSIFALEP GVVRYYLDPF HPKRKFIGVA LRRDLKLPSP
130 140 150 160 170 180
HFEPTVRRFG RFELTNKRAA YKEENSISRK DYLAKPNILK QLEVRESKRK ELQDKLSKVL
190 200 210 220 230 240
RDELKLDIKD IELATSYLIR VRASLKNGYP IEDARFNSRY YLKEEERLKA RRESWTNEKL
250 260 270 280 290 300
SESLSKIDEC SDLLNSSTSF NNKLELHQYI SEQEKQALKA KLLEDLEKSQ HLETKKDKNY
310 320 330 340 350 360
IKALFKDACN FLTLSEEVHL RRKYLKSVFP ETDSTVETKS GKKSIVSRRF DYTKNKVEVI
370
ARSRRAFLSK L
References and
documentation are available.
Number of amino acids: 371
Molecular weight: 43226.57
Theoretical pI: 9.82
Total number of negatively charged residues (Asp + Glu): 51
Total number of positively charged residues (Arg + Lys): 77
Atomic composition:
Carbon C 1926
Hydrogen H 3111
Nitrogen N 555
Oxygen O 564
Sulfur S 5
Formula: C
1926H
3111N
555O
564S
5
Total number of atoms: 6161
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 31985
Abs 0.1% (=1 g/l) 0.740, assuming all pairs of Cys residues form cystines
Ext. coefficient 31860
Abs 0.1% (=1 g/l) 0.737, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 50.47
This classifies the protein as unstable.
Aliphatic index: 80.16
Grand average of hydropathicity (GRAVY): -0.788