ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MWPLFLLGII GVVSAGIPGT NGDIRQPNFQ KELMDEAKVY LFKFGYMIPE PHQNVKTIQE 

        70         80         90        100        110        120 
PSEKQTKDAL IRFQSAFLYY SSGDVDVPTQ AKMNEWRCAN NDMDKGQPIP PASKKELWTK 

       130        140        150        160        170        180 
KSLTYKVVNT PNTLSQAQIR SAAHEAFEQW TRASGFKFVE TTGATPDITI TFYDVPQSNL 

       190        200        210        220        230        240 
RIAGSASKPV NSHIILDKNQ EWAYKSQAPM GISLYHTLLH EIGHILGLPH TFYRGSIMHP 

       250        260        270        280        290        300 
IFKPVLLPHG TVDTVPNVDR LAIRKIYGLS SVDHSTPSSD VDRSKCPKHL DSVVAINDQE 

       310        320        330        340        350        360 
WLFFRSNKVY KLNNRKFVDS GRPIQQVFPR GPQFVNATVS SGPLTLLFVE RTIYGYEYDG 

       370        380        390        400        410        420 
VQFTEAPNYP KELHDRVLFY PQGAFPLNNG SVILLSGNVF ATYNVQQNAP SFLNDKNRYF 

       430        440        450        460        470 
PNLPEDIKSG IEKTQGFTDA YYMFDEATVS DYDMNSKQVL QLQNIPDFLK CT 

References and documentation are available.

Number of amino acids: 472

Molecular weight: 53397.64

Theoretical pI: 7.20

Amino acid composition: 
Ala (A)  26	  5.5%
Arg (R)  17	  3.6%
Asn (N)  28	  5.9%
Asp (D)  27	  5.7%
Cys (C)   3	  0.6%
Gln (Q)  26	  5.5%
Glu (E)  20	  4.2%
Gly (G)  27	  5.7%
His (H)  12	  2.5%
Ile (I)  28	  5.9%
Leu (L)  37	  7.8%
Lys (K)  30	  6.4%
Met (M)   9	  1.9%
Phe (F)  27	  5.7%
Pro (P)  34	  7.2%
Ser (S)  34	  7.2%
Thr (T)  28	  5.9%
Trp (W)   6	  1.3%
Tyr (Y)  21	  4.4%
Val (V)  32	  6.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 47
Total number of positively charged residues (Arg + Lys): 47

Atomic composition:

Carbon      C	      2422
Hydrogen    H	      3707
Nitrogen    N	       637
Oxygen      O	       704
Sulfur      S	        12

Formula: C2422H3707N637O704S12
Total number of atoms: 7482

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    64415
Abs 0.1% (=1 g/l)   1.206, assuming all pairs of Cys residues form cystines


Ext. coefficient    64290
Abs 0.1% (=1 g/l)   1.204, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.30
This classifies the protein as stable.



Aliphatic index: 78.88

Grand average of hydropathicity (GRAVY): -0.386