ProtParam
User-provided sequence:
10 20 30 40 50 60
MWQCRRLAST SAAATVTQKQ RFRPVIGLEI HVQLNTLTKL FSRCPTSESS PNRRVDAFDM
70 80 90 100 110 120
ATPGILPALN RQCVLRALKM ARILHCQVPK SSRFDRKHYF YADMPAGYQI TQNEKPIAKN
130 140 150 160 170 180
GKFGFWVFDE RIEVPYWKEV EILQLQLEQD SGKTIHMTSQ AAPPTSLIDY NRAGCALVEI
190 200 210 220 230 240
VTSPCFTTAI EAIRFVQILR LLLIHHAICD GELHRGHIRV DANVSLAKDD VWGTRTEIKN
250 260 270 280 290 300
MNSIRTIHTA INYEIARQFE ILKNGGAVQN ETRAADSEGR TVSMRDKETE ETDYRFMVEP
310 320 330 340 350 360
NLPQLKIHGK WLKEVEEELK CEGKADFEVL REDVGFDARS AVHIAEEPEL LSFVKRCIRY
370 380 390 400 410 420
KAPTVTPVDI LYWMKELKTV MQRSKANYPP QSEFFARQFT TLLKFSSEHR LTRLRLLDLL
430 440 450 460 470 480
RHYATDDDVM AGELEILIEE NRYWRIRAPA EIDEIVRKSM EKQEKLVEKC RSGHVKSFNR
490 500
LRNSIIDSSN KCIELEDLEA SIRRLL
References and
documentation are available.
Number of amino acids: 506
Molecular weight: 58495.29
Theoretical pI: 8.51
Total number of negatively charged residues (Asp + Glu): 70
Total number of positively charged residues (Arg + Lys): 75
Atomic composition:
Carbon C 2590
Hydrogen H 4144
Nitrogen N 742
Oxygen O 755
Sulfur S 23
Formula: C
2590H
4144N
742O
755S
23
Total number of atoms: 8254
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 57005
Abs 0.1% (=1 g/l) 0.975, assuming all pairs of Cys residues form cystines
Ext. coefficient 56380
Abs 0.1% (=1 g/l) 0.964, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 54.50
This classifies the protein as unstable.
Aliphatic index: 88.68
Grand average of hydropathicity (GRAVY): -0.405