ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MWSPDEIASL CYNHFNKLPK RGKPESGREW TLLAAVIQIT DCPEQKTVQK QVVSLGTGTK 

        70         80         90        100        110        120 
CIGRSAMSMK GDVLNDSHAE VIARRGSVRY LTEQLLKAVR GQSSDVFCAG SEKGKWRLKA 

       130        140        150        160        170        180 
GVSFLFFTSQ TPCGDASIFP MSGSEAQPCE PVKETTQNEE SEPRGLKRPA DQETRQKTKH 

       190        200        210        220        230        240 
PKINTSDKQP SCRSSGDTED KAEAENKSCS ERSEAEPLDL HRTGAKSVPD GPKDLLQPGL 

       250        260        270        280        290        300 
LYHHTGLLRV KPGRGEPTLS LSCSDKLARW CILGFQGALL MHYLQEALYF SAVLVGKSPY 

       310        320        330        340        350        360 
SHPALRRALH TRCSHVKDLP SGFSFHDPEL LQSSLEFPHN HTHTKSKHTH SQGRISPCGA 

       370        380        390        400        410        420 
AISWCAVSQQ PLDVTANGYK QGVTKKALGT PQARSLICKV ELFHSFLKVV AATEDSELPE 

       430        440        450        460 
TLRGKDWKTY WDYKQAAGSY QLAWTQLRLQ AFPQWPRGPR QLLLFQ 

References and documentation are available.

Number of amino acids: 466

Molecular weight: 51748.79

Theoretical pI: 9.05

Amino acid composition: 
Ala (A)  34	  7.3%
Arg (R)  26	  5.6%
Asn (N)   8	  1.7%
Asp (D)  20	  4.3%
Cys (C)  14	  3.0%
Gln (Q)  28	  6.0%
Glu (E)  28	  6.0%
Gly (G)  33	  7.1%
His (H)  17	  3.6%
Ile (I)  11	  2.4%
Leu (L)  48	 10.3%
Lys (K)  35	  7.5%
Met (M)   5	  1.1%
Phe (F)  15	  3.2%
Pro (P)  30	  6.4%
Ser (S)  45	  9.7%
Thr (T)  28	  6.0%
Trp (W)   9	  1.9%
Tyr (Y)  10	  2.1%
Val (V)  22	  4.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 48
Total number of positively charged residues (Arg + Lys): 61

Atomic composition:

Carbon      C	      2280
Hydrogen    H	      3598
Nitrogen    N	       658
Oxygen      O	       682
Sulfur      S	        19

Formula: C2280H3598N658O682S19
Total number of atoms: 7237

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    65275
Abs 0.1% (=1 g/l)   1.261, assuming all pairs of Cys residues form cystines


Ext. coefficient    64400
Abs 0.1% (=1 g/l)   1.244, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 49.44
This classifies the protein as unstable.



Aliphatic index: 70.36

Grand average of hydropathicity (GRAVY): -0.575