ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MWTADEIAQL CYAHYIKLPK QGKPEPNREW TLLAAVVKIQ TPANQACDTP DKQIQVTKEV 

        70         80         90        100        110        120 
VSMGTGTKCI GQSKMRESGD ILNDSHAEVI ARRSFQRYIL HQLHVAAVLK EDSIFLPGTQ 

       130        140        150        160        170        180 
RGLWRLRPDL SFVFFSSHTP CGDASIIPKL EFEEQPCCPV IRNWANNSPG EGSETLEGSR 

       190        200        210        220        230        240 
NKRSCEGPDS PVAKKKRLGT PAGSLSDCVA HHGAQESGPA KPDASSSGLT KEELDAVNGT 

       250        260        270        280        290        300 
ASGSFKVVDI YRTGAKCVPG ETGDLRKPGA AYHQVGLLRV KPGRGDRTCS MSCSDKMARW 

       310        320        330        340        350        360 
NVLGCQGALL MHFLEKPIYL SAVVIGQCPY SQEAMRRALT GRCQETLVLP RGFEVQELKI 

       370        380        390        400        410        420 
QQSSLLFEQS RCAVHRKRGD SPGRLVPCGA AISWSAVPQQ PLDVTANGFP QGTTKKEIGS 

       430        440        450        460        470        480 
PRARSRISKV ELFRSFQKLL SCIAEDERPD SVRVQKLDTY QEYKEAASAY QEAWSALRRV 

       490 
QPFSSWIRNP PNYHQFK 

References and documentation are available.

Number of amino acids: 497

Molecular weight: 54911.56

Theoretical pI: 9.04

Amino acid composition: 
Ala (A)  41	  8.2%
Arg (R)  35	  7.0%
Asn (N)  12	  2.4%
Asp (D)  21	  4.2%
Cys (C)  17	  3.4%
Gln (Q)  30	  6.0%
Glu (E)  31	  6.2%
Gly (G)  37	  7.4%
His (H)  11	  2.2%
Ile (I)  21	  4.2%
Leu (L)  39	  7.8%
Lys (K)  31	  6.2%
Met (M)   7	  1.4%
Phe (F)  15	  3.0%
Pro (P)  33	  6.6%
Ser (S)  44	  8.9%
Thr (T)  22	  4.4%
Trp (W)   8	  1.6%
Tyr (Y)  11	  2.2%
Val (V)  31	  6.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 52
Total number of positively charged residues (Arg + Lys): 66

Atomic composition:

Carbon      C	      2404
Hydrogen    H	      3827
Nitrogen    N	       705
Oxygen      O	       721
Sulfur      S	        24

Formula: C2404H3827N705O721S24
Total number of atoms: 7681

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    61390
Abs 0.1% (=1 g/l)   1.118, assuming all pairs of Cys residues form cystines


Ext. coefficient    60390
Abs 0.1% (=1 g/l)   1.100, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.13
This classifies the protein as unstable.



Aliphatic index: 73.42

Grand average of hydropathicity (GRAVY): -0.478