ProtParam
User-provided sequence:
10 20 30 40 50 60
MWTFNRSTAE APTMPSSSNT VGGNIYFLEE EYRKKRFRQN SKCIFKIFSS NYQVRLLFSS
70 80 90 100 110 120
FAVKNRLFFV KKSLKMADND RKRKHNRWNQ KVKQKSVEAG VTGLFFSCEG HEKQALQEAY
130 140 150 160 170 180
SIIDELLEDP ANGISLKAPE AAPQGSEAPG NPAEAEDSDE DIADALKKAC DDQRQPKPGN
190 200 210 220 230 240
FVKKERRCIQ RPTGVKNCIF VSVKNANIQL LAEKMVELTQ KAPRCRFLQR VYPVEHTLAV
250 260 270 280 290 300
DLSKMNEVLM KVISDTLKAD GTGKLPTYSV EFKARNNDSV AKNSVLQMVD DAVCALAPTA
310 320 330 340
RVSLNHADVT FFVQVSRTTI MVGVCRQFYD RRKYSLRATP AAAAT
References and
documentation are available.
Number of amino acids: 345
Molecular weight: 38809.44
Theoretical pI: 9.42
Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 54
Atomic composition:
Carbon C 1707
Hydrogen H 2735
Nitrogen N 491
Oxygen O 510
Sulfur S 16
Formula: C
1707H
2735N
491O
510S
16
Total number of atoms: 5459
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 23420
Abs 0.1% (=1 g/l) 0.603, assuming all pairs of Cys residues form cystines
Ext. coefficient 22920
Abs 0.1% (=1 g/l) 0.591, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 42.61
This classifies the protein as unstable.
Aliphatic index: 74.09
Grand average of hydropathicity (GRAVY): -0.474