ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MYEGKKTKNM FLTRALEKIL ADKEVKKAHH SQLRKACEVA LEEIKAETEK QSPPHGEAKA 

        70         80         90        100        110        120 
GSSTLPPVKS KTNFIEADKY FLPFELACQS KCPRIVSTSL DCLQKLIAYG HLTGNAPDST 

       130        140        150        160        170        180 
TPGKKLIDRI IETICGCFQG PQTDEGVQLQ IIKALLTAVT SQHIEIHEGT VLQAVRTCYN 

       190        200        210        220        230        240 
IYLASKNLIN QTTAKATLTQ MLNVIFARME NQALQEAKQM EKERHRQHHH LLQSPVSHHE 

       250        260        270        280        290        300 
PESPQLRYLP PQTVDHIPQE HEGDLDPQTN DVDKSLQDDT EPENGSDISS AENEQTEADQ 

       310        320        330        340        350        360 
ATAAETLSKN DILYDGENHD CEEKPQDIVQ SIVEEMVNIV VGDTGERTTI NVSADGNNGT 

       370        380        390        400        410        420 
IEDGSDSENI QANGIPGTPI SVAYTPSLPD DRLSVSSNDT QESGNSSGPS PGAKFSHILQ 

       430        440        450        460        470        480 
KDAFLVFRSL CKLSMKPLSD GPPDPKSHEL RSKILSLQLL LSILQNAGPI FGTNEMFINA 

       490        500        510        520        530        540 
IKQYLCVALS KNGVSSVPEV FELSLSIFLT LLSNFKTHLK MQIEVFFKEI FLYILETSTS 

       550        560        570        580        590        600 
SFDHKWMVIQ TLTRICADAQ SVVDIYVNYD CDLNAANIFE RLVNDLSKIA QGRGSQELGM 

       610        620        630        640        650        660 
SNVQELSLRK KGLECLVSIL KCMVEWSKDQ YVNPNSQTTL GQEKPSEQET SEMKHPETIN 

       670        680        690        700        710        720 
RYGSLNSLES TSSSGIGSYS TQMSGTDNPE QFEVLKQQKE IIEQGIDLFT KKPKRGIQYL 

       730        740        750        760        770        780 
QEQGMLGTTP EDIAQFLHQE ERLDSTQVGE FLGDNDKFNK EVMYAYVDQH DFSGKDFVSA 

       790        800        810        820        830        840 
LRMFLEGFRL PGEAQKIDRL MEKFAARYLE CNQGQTLFAS ADTAYVLAYS IIMLTTDLHS 

       850        860        870        880        890        900 
PQVKNKMTKE QYIKMNRGIN DSKDLPEEYL SAIYNEIAGK KISMKETKEL TIPAKSSKQN 

       910        920        930        940        950        960 
VASEKQRRLL YNLEMEQMAK TAKALMEAVS HVQAPFTSAT HLEHVRPMFK LAWTPFLAAF 

       970        980        990       1000       1010       1020 
SVGLQDCDDT EVASLCLEGI RCAIRIACIF SIQLERDAYV QALARFTLLT VSSGITEMKQ 

      1030       1040       1050       1060       1070       1080 
KNIDTIKTLI TVAHTDGNYL GNSWHEILKC ISQLELAQLI GTGVKPRYIS GTVRGREGSL 

      1090       1100       1110       1120       1130       1140 
TGAKDQAPDE FVGLGLVGGN VDWKQIASIQ ESIGETSSQS VVVAVDRIFT GSTRLDGNAI 

      1150       1160       1170       1180       1190       1200 
VDFVRWLCAV SMDELLSTTH PRMFSLQKIV EISYYNMGRI RLQWSRIWEV IGDHFNKVGC 

      1210       1220       1230       1240       1250       1260 
NPNEDVAIFA VDSLRQLSMK FLEKGELANF RFQKDFLRPF EHIMKRNRSP TIRDMVVRCI 

      1270       1280       1290       1300       1310       1320 
AQMVNSQAAN IRSGWKNIFS VFHLAASDQD ESIVELAFQT TGHIVTLVFE KHFPATIDSF 

      1330       1340       1350       1360       1370       1380 
QDAVKCLSEF ACNAAFPDTS MEAIRLIRHC AKYVSDRPQA FKEYTSDDMN VAPEDRVWVR 

      1390       1400       1410       1420       1430       1440 
GWFPILFELS CIINRCKLDV RTRGLTVMFE IMKTYGYTYE KHWWQDLFRI VFRIFDNMKL 

      1450       1460       1470       1480       1490       1500 
PEQQTEKAEW MTTTCNHALY AICDVFTQYL EVLSDVLLDD IFAQLYWCVQ QDNEQLARSG 

      1510       1520       1530       1540       1550       1560 
TNCLENVVIL NGEKFTLEIW DKTCNCTLDI FKTTIPHALL TWRPISGETA PPTPSPVSEN 

      1570       1580       1590       1600       1610       1620 
QLDTISQKSV DIHDSIQPRS ADNRQQAPLA SVSTVNEEIS KIKPTAKFPE QKLFAALLIK 

      1630       1640       1650       1660       1670       1680 
CVVQLELIQT IDNIVFFPAT SRKEDAENLA AAQRDAVDFD VRVDTQDQGM YRFLTSQQLF 

      1690       1700       1710       1720       1730       1740 
KLLDCLLESH RFAKAFNSNN EQRTALWKAG FKGKSKPNLL KQETSSLACG LRILFRMYTD 

      1750       1760       1770       1780       1790       1800 
ESRASAWEEV QQRLLNVCSE ALSYFLTLTS ESHREAWTNL LLLFLTKVLK ISDNRFKAHA 

      1810       1820       1830       1840 
SFYYPLLCEI MQFDLIPELR AVLRRFFLRI GVVFQISQPP EQELGINKQ 

References and documentation are available.

Number of amino acids: 1849

Molecular weight: 208711.80

Theoretical pI: 5.52

Amino acid composition: 
Ala (A) 125	  6.8%
Arg (R)  80	  4.3%
Asn (N)  82	  4.4%
Asp (D) 102	  5.5%
Cys (C)  39	  2.1%
Gln (Q) 113	  6.1%
Glu (E) 137	  7.4%
Gly (G)  84	  4.5%
His (H)  42	  2.3%
Ile (I) 122	  6.6%
Leu (L) 182	  9.8%
Lys (K) 114	  6.2%
Met (M)  43	  2.3%
Phe (F)  85	  4.6%
Pro (P)  71	  3.8%
Ser (S) 144	  7.8%
Thr (T) 116	  6.3%
Trp (W)  20	  1.1%
Tyr (Y)  43	  2.3%
Val (V) 105	  5.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 239
Total number of positively charged residues (Arg + Lys): 194

Atomic composition:

Carbon      C	      9249
Hydrogen    H	     14618
Nitrogen    N	      2502
Oxygen      O	      2826
Sulfur      S	        82

Formula: C9249H14618N2502O2826S82
Total number of atoms: 29277

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   176445
Abs 0.1% (=1 g/l)   0.845, assuming all pairs of Cys residues form cystines


Ext. coefficient   174070
Abs 0.1% (=1 g/l)   0.834, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.82
This classifies the protein as unstable.



Aliphatic index: 87.35

Grand average of hydropathicity (GRAVY): -0.298