ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MYEYCSVVIK KYSKYTIPSF APNGFQSMLE PPQIDKWQHL SANCTLQFRV LLMDSRQILI 

        70         80         90        100        110        120 
NVVLNNSTLL ENIRLPLGDN QDLIQFSCKS PIISCKYISE EFGPRMLRRF QMNLPNDVEF 

       130        140        150        160        170        180 
NRTVVSLKNL NFVLRTARTS IAQSTITSQV QGNNNGTKVC FTEGPKVSSY TNPNTQFQTQ 

       190        200        210        220        230        240 
NMIMDFSQRY QEESERESNN RSNITLPHDS IQIAQQIWPN TDLNVVQSSQ DLNTPMATQT 

       250        260        270        280        290        300 
VLGRPESLIV QPLEVSQSPP NTTNCLPNAE NKKKKVDTTS DFTSRKEIAL CKTGLLETIH 

       310        320        330        340        350        360 
IPKERESQMQ SVTGLDATPT IIWSPGKDNT AKKNTSNKKN IDDKLTNPQK SGNTHTPDRN 

       370        380        390        400        410        420 
KEVLPNGTLN ETRKEASPSE GLTIRVKNVN RNASRKISKR LIKEKLKDEE FMKWVNKVET 


VLNKMFEK 

References and documentation are available.

Number of amino acids: 428

Molecular weight: 48706.37

Theoretical pI: 9.26

Amino acid composition: 
Ala (A)  12	  2.8%
Arg (R)  21	  4.9%
Asn (N)  42	  9.8%
Asp (D)  17	  4.0%
Cys (C)   7	  1.6%
Gln (Q)  27	  6.3%
Glu (E)  27	  6.3%
Gly (G)  13	  3.0%
His (H)   4	  0.9%
Ile (I)  27	  6.3%
Leu (L)  35	  8.2%
Lys (K)  35	  8.2%
Met (M)  11	  2.6%
Phe (F)  14	  3.3%
Pro (P)  25	  5.8%
Ser (S)  38	  8.9%
Thr (T)  37	  8.6%
Trp (W)   4	  0.9%
Tyr (Y)   7	  1.6%
Val (V)  25	  5.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 44
Total number of positively charged residues (Arg + Lys): 56

Atomic composition:

Carbon      C	      2121
Hydrogen    H	      3437
Nitrogen    N	       607
Oxygen      O	       668
Sulfur      S	        18

Formula: C2121H3437N607O668S18
Total number of atoms: 6851

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    32805
Abs 0.1% (=1 g/l)   0.674, assuming all pairs of Cys residues form cystines


Ext. coefficient    32430
Abs 0.1% (=1 g/l)   0.666, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.52
This classifies the protein as unstable.



Aliphatic index: 76.24

Grand average of hydropathicity (GRAVY): -0.689