ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MYFDKDNSMS PRPLLPSDEQ KLNINLLTKK EKFSHLDPHY DAKATPQRST SNRNVGDLLL 

        70         80         90        100        110        120 
EKRTAKPMIQ KALTNTDNFI EMYHNQQRKN LDDDTIKEVM INDENGKTVA STNDGRYDND 

       130        140        150        160        170        180 
YDNNDINDQK TLDNIAGSPH MEKNRNKVKI EHDSSSQKPI AKESSKAQKN IIKKGIKDFK 

       190        200        210        220        230        240 
FGSVIGDGAY STVMLATSID TKKRYAAKVL NKEYLIRQKK VKYVSIEKTA LQKLNNSPSV 

       250        260        270        280        290        300 
VRLFSTFQDE SSLYFLLEYA PNGDFLSLMK KYGSLDETCA RYYAAQIIDA IDYLHSNGII 

       310        320        330        340        350        360 
HRDIKPENIL LDGEMKIKLT DFGTAKLLNP TNNSVSKPEY DLSTRSKSFV GTAEYVSPEL 

       370        380        390        400        410        420 
LNDSFTDYRC DIWAFGCILF QMIAGKPPFK ATNEYLTFQK VMKVQYAFTP GFPLIIRDLV 

       430        440        450        460        470        480 
KKILVKNLDR RLTISQIKEH HFFKDLNFKD GSVWSKTPPE IKPYKINAKS MQAMPSGSDR 

       490        500        510        520        530        540 
KLVKKSVNTL GKSHLVTQRS ASSPSVEETT HSTLYNNNTH ASTESEISIK KRPTDERTAQ 

       550        560        570        580        590        600 
ILENARKGIN NRKNQPGKRT PSGAASAALA ASAALTKKTM QSYPTSSSKS SRSSSPATTS 

       610        620        630        640        650        660 
RPGTYKRTSS TESKPFAKSP PLSASVLSSK VPMPPYTPPM SPPMTPYDTY QMTPPYTTKQ 

       670        680        690        700        710        720 
QDYSDTAIAA PKPCISKQNV KNSTDSPLMN KQDIQWSFYL KNINEHVLRT EKLDFVTTNY 

       730        740        750        760        770        780 
DILEKKMLKL NGSLLDPQLF GKPRHTFLSQ VARSGGEVTG FRNDPTMTAY SKTEDTYYSK 

       790        800        810        820        830        840 
NIIDLQLLED DYRIEGGDLS ELLTNRSGEG YKCNQNSSPM KDDDKSESNN KGSSVFSGKI 

       850        860        870        880        890        900 
KKLFHPTSAA ETLSSSDEKT KYYKRTIVMT SFGRFLVFAK RRQPNPVTNL KYELEYDINL 

       910        920        930        940        950        960 
RQQGTKIKEL IIPLEMGTNH IVVIQTPYKS FLLSTDKKTT SKLFTVLKKI LNSNTNKIEK 

       970        980        990       1000       1010       1020 
ELLQRNQKVI ERRTSSSGRA IPKDLPTSKS PSPKPRTHSQ SPSISKHNSF SESINSAKSN 

      1030       1040       1050       1060       1070       1080 
RSSRIFETFI NAKEQNSKKH AAPVPLTSKL VNGLPKRQVT VGLGLNTGTN FKNSSAKSKR 


S 

References and documentation are available.

Number of amino acids: 1081

Molecular weight: 121660.18

Theoretical pI: 9.65

Amino acid composition: 
Ala (A)  55	  5.1%
Arg (R)  47	  4.3%
Asn (N)  73	  6.8%
Asp (D)  61	  5.6%
Cys (C)   5	  0.5%
Gln (Q)  40	  3.7%
Glu (E)  51	  4.7%
Gly (G)  43	  4.0%
His (H)  19	  1.8%
Ile (I)  62	  5.7%
Leu (L)  88	  8.1%
Lys (K) 120	 11.1%
Met (M)  24	  2.2%
Phe (F)  38	  3.5%
Pro (P)  63	  5.8%
Ser (S) 121	 11.2%
Thr (T)  85	  7.9%
Trp (W)   3	  0.3%
Tyr (Y)  41	  3.8%
Val (V)  42	  3.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 112
Total number of positively charged residues (Arg + Lys): 167

Atomic composition:

Carbon      C	      5365
Hydrogen    H	      8614
Nitrogen    N	      1496
Oxygen      O	      1666
Sulfur      S	        29

Formula: C5365H8614N1496O1666S29
Total number of atoms: 17170

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    77840
Abs 0.1% (=1 g/l)   0.640, assuming all pairs of Cys residues form cystines


Ext. coefficient    77590
Abs 0.1% (=1 g/l)   0.638, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.53
This classifies the protein as unstable.



Aliphatic index: 70.47

Grand average of hydropathicity (GRAVY): -0.744