ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MYFWYLMPVY MPHWPKPLGA RPTEIDLKLH RVTRVLKRLG SPHLSVPPVI HVAGTNGKGS 

        70         80         90        100        110        120 
TIAFLRAILQ ASGYSVNVYT SPHLRYFNER IVLRNREISD KFLYDVLEDV RIASQGEKLT 

       130        140        150        160        170        180 
FFEGTTLAAI LAFSRSNADF SLIETGMGGR LDATNVFPKP IATIITAIDF DHTSYLGATI 

       190        200        210        220        230        240 
AQIAYEKAGI IKEGVPCVVA RQHGEAIDII QGVAKSKRVP VFRQGHEWEV GTDGRMLQFG 

       250        260        270        280        290        300 
VSSHVEEYPL PNLRGLHQIG NAGNAIAAAT ILSSRYNCDA ISVESVSIGI KNADWPARLQ 

       310        320        330        340        350        360 
LIRGKKNKLV GMLPEGWEFY MDGAHNPSGA KTISEWLEKE KIHIILGMTR DKDTKEFVSH 

       370        380        390        400        410        420 
LLPNIESISV VCVQGEPRSK TIEEIAQPIL ELGLECRKSS SLEDAFYHIL SRDSDSKKIL 

       430        440 
LCGSLFLFRD ICSYGEIEGC LC 

References and documentation are available.

Number of amino acids: 442

Molecular weight: 49112.63

Theoretical pI: 7.22

Amino acid composition: 
Ala (A)  32	  7.2%
Arg (R)  25	  5.7%
Asn (N)  14	  3.2%
Asp (D)  19	  4.3%
Cys (C)   8	  1.8%
Gln (Q)  11	  2.5%
Glu (E)  30	  6.8%
Gly (G)  35	  7.9%
His (H)  14	  3.2%
Ile (I)  40	  9.0%
Leu (L)  42	  9.5%
Lys (K)  24	  5.4%
Met (M)   8	  1.8%
Phe (F)  17	  3.8%
Pro (P)  21	  4.8%
Ser (S)  34	  7.7%
Thr (T)  20	  4.5%
Trp (W)   6	  1.4%
Tyr (Y)  14	  3.2%
Val (V)  28	  6.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 49
Total number of positively charged residues (Arg + Lys): 49

Atomic composition:

Carbon      C	      2209
Hydrogen    H	      3492
Nitrogen    N	       600
Oxygen      O	       634
Sulfur      S	        16

Formula: C2209H3492N600O634S16
Total number of atoms: 6951

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    54360
Abs 0.1% (=1 g/l)   1.107, assuming all pairs of Cys residues form cystines


Ext. coefficient    53860
Abs 0.1% (=1 g/l)   1.097, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.00
This classifies the protein as unstable.



Aliphatic index: 97.96

Grand average of hydropathicity (GRAVY): -0.056