ProtParam
User-provided sequence:
10 20 30 40 50 60
MYGGDEVSAI VVDLGSHTCK AGYAGEDAPK AVFPSVIGAV DGVEAMDVDV DSTKTNSNSE
70 80 90 100 110 120
DSKTESEKEK SKRKLYVGSQ AMSYRRDHME VLSPIKDGIV SDWDLVDNIW EHAFKSCLMI
130 140 150 160 170 180
DPTEHPMLLA EPPLNTQQQR EKAAELMFEK YKVPALFMAK NPVLTSFATG RATSLVVDCG
190 200 210 220 230 240
GGSTTISPVH DGYVLQKAVV SSPLGGEFLT DCLLKSLESK GIKIRPRYSF KRKEVRAGEF
250 260 270 280 290 300
QVEDVDIPDT TESYKLFCQR MIVGDIKDSI CRVPDTPYDD KSYSNIPTTS YELPDGQTLE
310 320 330 340 350 360
IGADRFKVPD VMFNPSIVQT IPGMEKYAEM IPSVRGLPHM VMESINKCDV DIRRELYSSI
370 380 390 400 410 420
LLAGGTSSMQ QLKERLEKDL IEESPHSARV KVLASGNTTE RRFSVWIGGS ILASLGSFQQ
430 440
MWFSKSEYEE HGASYIQRKC P
References and
documentation are available.
Number of amino acids: 441
Molecular weight: 48936.61
Theoretical pI: 5.20
Total number of negatively charged residues (Asp + Glu): 65
Total number of positively charged residues (Arg + Lys): 51
Atomic composition:
Carbon C 2152
Hydrogen H 3407
Nitrogen N 575
Oxygen O 677
Sulfur S 24
Formula: C
2152H
3407N
575O
677S
24
Total number of atoms: 6835
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 44850
Abs 0.1% (=1 g/l) 0.916, assuming all pairs of Cys residues form cystines
Ext. coefficient 44350
Abs 0.1% (=1 g/l) 0.906, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 36.28
This classifies the protein as stable.
Aliphatic index: 78.21
Grand average of hydropathicity (GRAVY): -0.357