ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MYGRDPWGGP LEINTADSAT DDDRSRNLND LDRAALSRPL DETQQSWLLG PTEQKKKKYV 

        70         80         90        100        110        120 
DLGCIIVSRK IFVWTVGTLV AAALLAGFIT LIVKTVPRHH PKTPPPDNYT IALHKALKFF 

       130        140        150        160        170        180 
NAQKSGKLPK HNNVSWRGNS GLQDGKGETG SFYKDLVGGY YDAGDAIKFN FPMAYAMTML 

       190        200        210        220        230        240 
SWSVIEYSAK YEAAGELTHV KELIKWGTDY FLKTFNSTAD SIDDLVSQVG SGNTDDGNTD 

       250        260        270        280        290        300 
PNDHYCWMRP EDMDYKRPVT TCNGGCSDLA AEMAAALASA SIVFKDNKEY SKKLVHGAKV 

       310        320        330        340        350        360 
VYQFGRTRRG RYSAGTAESS KFYNSSMYWD EFIWGGAWMY YATGNVTYLN LITQPTMAKH 

       370        380        390        400        410        420 
AGAFWGGPYY GVFSWDNKLA GAQLLLSRLR LFLSPGYPYE EILRTFHNQT SIVMCSYLPI 

       430        440        450        460        470        480 
FNKFNRTNGG LIELNHGAPQ PLQYSVNAAF LATLYSDYLD AADTPGWYCG PNFYSTSVLR 

       490        500        510        520        530        540 
DFARSQIDYI LGKNPRKMSY VVGFGTKYPR HVHHRGASIP KNKVKYNCKG GWKWRDSKKP 

       550        560        570        580        590        600 
NPNTIEGAMV AGPDKRDGYR DVRMNYNYTE PTLAGNAGLV AALVALSGEE EATGKIDKNT 

       610        620 
IFSAVPPLFP TPPPPPAPWK P 

References and documentation are available.

Number of amino acids: 621

Molecular weight: 69191.36

Theoretical pI: 8.96

Amino acid composition: 
Ala (A)  54	  8.7%
Arg (R)  27	  4.3%
Asn (N)  35	  5.6%
Asp (D)  38	  6.1%
Cys (C)   7	  1.1%
Gln (Q)  13	  2.1%
Glu (E)  21	  3.4%
Gly (G)  54	  8.7%
His (H)  13	  2.1%
Ile (I)  24	  3.9%
Leu (L)  49	  7.9%
Lys (K)  43	  6.9%
Met (M)  14	  2.3%
Phe (F)  25	  4.0%
Pro (P)  40	  6.4%
Ser (S)  40	  6.4%
Thr (T)  39	  6.3%
Trp (W)  16	  2.6%
Tyr (Y)  37	  6.0%
Val (V)  32	  5.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 59
Total number of positively charged residues (Arg + Lys): 70

Atomic composition:

Carbon      C	      3129
Hydrogen    H	      4739
Nitrogen    N	       835
Oxygen      O	       904
Sulfur      S	        21

Formula: C3129H4739N835O904S21
Total number of atoms: 9628

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   143505
Abs 0.1% (=1 g/l)   2.074, assuming all pairs of Cys residues form cystines


Ext. coefficient   143130
Abs 0.1% (=1 g/l)   2.069, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.23
This classifies the protein as stable.



Aliphatic index: 69.48

Grand average of hydropathicity (GRAVY): -0.439