ProtParam
User-provided sequence:
10 20 30 40 50 60
MYIIYGADHR ISNCSLLKRR IPEARIFKLY PNDKTSRSSS ASVAIPDYET QGQTAGQITP
70 80 90 100 110 120
KSWLCMLSST VPATKSSEWI FDTGCTSHMC HDRSIFSSFT RSSRKDFVRG VGGSIPIMGS
130 140
GTVNIGTVQL HDVSYVPDLP VNLISV
References and
documentation are available.
Number of amino acids: 146
Molecular weight: 16049.30
Theoretical pI: 9.00
Total number of negatively charged residues (Asp + Glu): 11
Total number of positively charged residues (Arg + Lys): 15
Atomic composition:
Carbon C 705
Hydrogen H 1117
Nitrogen N 197
Oxygen O 215
Sulfur S 8
Formula: C
705H
1117N
197O
215S
8
Total number of atoms: 2242
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 18700
Abs 0.1% (=1 g/l) 1.165, assuming all pairs of Cys residues form cystines
Ext. coefficient 18450
Abs 0.1% (=1 g/l) 1.150, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 46.11
This classifies the protein as unstable.
Aliphatic index: 80.07
Grand average of hydropathicity (GRAVY): -0.139