ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MYIKAEQKPQ QFERKNEKLD RNKNQQLPDL ETDFKGYRVN SDLYNKERDG STEETLNSLK 

        70         80         90        100        110        120 
FLHKPQRVTQ MRANRFPEEE VQRNTDLNKR IFSAGNDENV DNESGWSKIA AAKNHTSVES 

       130        140        150        160        170        180 
LNGSTRPPFK IELPPLSPKS TVPKSFQAEY PEAKSPGNDM NFEYDEEILI PFAPPVYKKS 

       190        200        210        220        230        240 
GELLKSSLKR RSKSLPTTPG IRSGNGVQAR DGSPMLIRSK SVHFDQAAPV KYFAEDESPI 

       250        260        270        280        290        300 
NVNKTEQHDN CLSFKHKPVN LMVDPEEETK MLSSGLETTS IDDDLTTVAP KGFAHPAKIS 

       310        320        330        340        350        360 
NPNNGKGTNN TKLRKSKRFQ NLLKNRTDMP PSKSNKKFVN GGGAHEISDR NSKNYHVVGL 

       370        380        390        400        410        420 
YSKNFPILSN KNPKSLKLNI FINLSQNKKV FLQELSLYIH RDNNYFSNSS SFYNIPNSHN 

       430        440        450        460        470        480 
GNDCNGVAKG YNAGCTRLIA GRILVKNIFY DKRVVVRYTW DSWRTTHEVE CVYISDGDGI 

       490        500        510        520        530        540 
LPGTNMDIFH FIIDDVSKVD PRGKLEFCIH YSTRNDYERE EYWDNNNGNN YKVDVVMDGF 


NDPFAAAA 

References and documentation are available.

Number of amino acids: 548

Molecular weight: 62318.74

Theoretical pI: 8.87

Amino acid composition: 
Ala (A)  24	  4.4%
Arg (R)  28	  5.1%
Asn (N)  56	 10.2%
Asp (D)  35	  6.4%
Cys (C)   5	  0.9%
Gln (Q)  15	  2.7%
Glu (E)  35	  6.4%
Gly (G)  30	  5.5%
His (H)  13	  2.4%
Ile (I)  26	  4.7%
Leu (L)  37	  6.8%
Lys (K)  50	  9.1%
Met (M)   9	  1.6%
Phe (F)  26	  4.7%
Pro (P)  33	  6.0%
Ser (S)  47	  8.6%
Thr (T)  25	  4.6%
Trp (W)   4	  0.7%
Tyr (Y)  20	  3.6%
Val (V)  30	  5.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 70
Total number of positively charged residues (Arg + Lys): 78

Atomic composition:

Carbon      C	      2744
Hydrogen    H	      4279
Nitrogen    N	       783
Oxygen      O	       852
Sulfur      S	        14

Formula: C2744H4279N783O852S14
Total number of atoms: 8672

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    52050
Abs 0.1% (=1 g/l)   0.835, assuming all pairs of Cys residues form cystines


Ext. coefficient    51800
Abs 0.1% (=1 g/l)   0.831, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.69
This classifies the protein as unstable.



Aliphatic index: 65.09

Grand average of hydropathicity (GRAVY): -0.869