ProtParam
User-provided sequence:
10 20 30 40 50 60
MYKYTSINKS DKIYETHNME EKKKKGNNNN FSGLLNNEID DNNKKEKLKN SISKMYSNHK
70 80 90 100 110 120
NRENFNECEK EDLVVIDEKD NNKKKKKNMT NTFEQDNNYN MNDNKRLGSF FKINDKCESI
130 140 150 160 170 180
NENVNNINKQ SLKDSILFDN INEKEYFNET KEENKEGNKS NDIEKINCMK VKKKTVKKNK
190 200 210 220 230 240
KKINKIINNK NKISKSNDIE EQIINISKHI YKILNISKLP KCDILKRTNR RYAETFLYLT
250 260 270 280 290 300
NGYNLDIEQI IKRSLYKRMY KNNSIIKVTG IHIYSLCKHH LLPFEGTCDI EYIPNKYIIG
310 320 330 340 350 360
LSKFSRIVDV FSRRLQLQED LTNDICNALK KYLKPLYIKV SIVAKHLCIN MRGVKEHDAK
370 380
TITYASYKAE KENPTVHSLN IDSSVENLN
References and
documentation are available.
Number of amino acids: 389
Molecular weight: 45876.53
Theoretical pI: 9.39
Total number of negatively charged residues (Asp + Glu): 52
Total number of positively charged residues (Arg + Lys): 75
Atomic composition:
Carbon C 2017
Hydrogen H 3268
Nitrogen N 568
Oxygen O 618
Sulfur S 16
Formula: C
2017H
3268N
568O
618S
16
Total number of atoms: 6487
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 28810
Abs 0.1% (=1 g/l) 0.628, assuming all pairs of Cys residues form cystines
Ext. coefficient 28310
Abs 0.1% (=1 g/l) 0.617, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 49.68
This classifies the protein as unstable.
Aliphatic index: 80.41
Grand average of hydropathicity (GRAVY): -0.973