ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MYNSSTNHHE GAPTSGHGYY MSQQQDQQHQ QQQQYANEMN PYQQIPRPPA AGFSSNYMKE 

        70         80         90        100        110        120 
QGSHQSLQEH LQRETGNLGS GFTDVPALNY PATPPPHNNY AASNQMINTP PPSMGGLYRH 

       130        140        150        160        170        180 
NNNSQSMVQN GNGSGNAQLP QLSPGQYSIE SEYNQNLNGS SSSSPFHQPQ TLRSNGSYSS 

       190        200        210        220        230        240 
GLRSVKSFQR LQQEQENVQV QQQLSQAQQQ NSRQQQQQLQ YQQQQQQQQQ QQHMQIQQQQ 

       250        260        270        280        290        300 
QQQQQQQQSQ SPVQSGFNNG TISNYMYFER RPDLLTKGTQ DKAAAVKLKI ENFYQSSVKY 

       310        320        330        340        350        360 
AIERNERRVE LETELTSHNW SEERKSRQLS SLGKKESQFL RLRRTRLSLE DFHTVKVIGK 

       370        380        390        400        410        420 
GAFGEVRLVQ KKDTGKIYAM KTLLKSEMYK KDQLAHVKAE RDVLAGSDSP WVVSLYYSFQ 

       430        440        450        460        470        480 
DAQYLYLIME FLPGGDLMTM LIRWQLFTED VTRFYMAECI LAIETIHKLG FIHRDIKPDN 

       490        500        510        520        530        540 
ILIDIRGHIK LSDFGLSTGF HKTHDSNYYK KLLQQDEATN GISKPGTYNA NTTDTANKRQ 

       550        560        570        580        590        600 
TMVVDSISLT MSNRQQIQTW RKSRRLMAYS TVGTPDYIAP EIFLYQGYGQ ECDWWSLGAI 

       610        620        630        640        650        660 
MYECLIGWPP FCSETPQETY RKIMNFEQTL QFPDDIHISY EAEDLIRRLL THADQRLGRH 

       670        680        690        700        710        720 
GGADEIKSHP FFRGVDWNTI RQVEAPYIPK LSSITDTRFF PTDELENVPD SPAMAQAAKQ 

       730        740        750 
REQMTKQGGS APVKEDLPFI GYTYSRFDYL TRKNAL 

References and documentation are available.

Number of amino acids: 756

Molecular weight: 86945.86

Theoretical pI: 7.72

Amino acid composition: 
Ala (A)  38	  5.0%
Arg (R)  40	  5.3%
Asn (N)  40	  5.3%
Asp (D)  33	  4.4%
Cys (C)   4	  0.5%
Gln (Q)  95	 12.6%
Glu (E)  42	  5.6%
Gly (G)  46	  6.1%
His (H)  22	  2.9%
Ile (I)  36	  4.8%
Leu (L)  58	  7.7%
Lys (K)  36	  4.8%
Met (M)  22	  2.9%
Phe (F)  27	  3.6%
Pro (P)  37	  4.9%
Ser (S)  66	  8.7%
Thr (T)  43	  5.7%
Trp (W)   8	  1.1%
Tyr (Y)  38	  5.0%
Val (V)  25	  3.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 75
Total number of positively charged residues (Arg + Lys): 76

Atomic composition:

Carbon      C	      3810
Hydrogen    H	      5887
Nitrogen    N	      1099
Oxygen      O	      1189
Sulfur      S	        26

Formula: C3810H5887N1099O1189S26
Total number of atoms: 12011

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   100870
Abs 0.1% (=1 g/l)   1.160, assuming all pairs of Cys residues form cystines


Ext. coefficient   100620
Abs 0.1% (=1 g/l)   1.157, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 66.34
This classifies the protein as unstable.



Aliphatic index: 63.11

Grand average of hydropathicity (GRAVY): -0.873