ProtParam
User-provided sequence:
10 20 30 40 50 60
MYPGSGRYTY NNAGGNNGYQ RPMAPPPNQQ YGQQYGQQYE QQYGQQYGQQ NDQQFSQQYA
70 80 90 100 110 120
PPPGPPPMAY NRPVYPPPQF QQEQAKAQLS NGYNNPNVNA SNMYGPPQNM SLPPPQTQTI
130 140 150 160 170 180
QGTDQPYQYS QCTGRRKALI IGINYIGSKN QLRGCINDAH NIFNFLTNGY GYSSDDIVIL
190 200 210 220 230 240
TDDQNDLVRV PTRANMIRAM QWLVKDAQPN DSLFLHYSGH GGQTEDLDGD EEDGMDDVIY
250 260 270 280 290 300
PVDFETQGPI IDDEMHDIMV KPLQQGVRLT ALFDSCHSGT VLDLPYTYST KGIIKEPNIW
310 320 330 340 350 360
KDVGQDGLQA AISYATGNRA ALIGSLGSIF KTVKGGMGNN VDRERVRQIK FSAADVVMLS
370 380 390 400 410 420
GSKDNQTSAD AVEDGQNTGA MSHAFIKVMT LQPQQSYLSL LQNMRKELAG KYSQKPQLSS
430
SHPIDVNLQF IM
References and
documentation are available.
Number of amino acids: 432
Molecular weight: 47982.34
Theoretical pI: 5.16
Total number of negatively charged residues (Asp + Glu): 41
Total number of positively charged residues (Arg + Lys): 31
Atomic composition:
Carbon C 2097
Hydrogen H 3223
Nitrogen N 589
Oxygen O 668
Sulfur S 19
Formula: C
2097H
3223N
589O
668S
19
Total number of atoms: 6596
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 49865
Abs 0.1% (=1 g/l) 1.039, assuming all pairs of Cys residues form cystines
Ext. coefficient 49740
Abs 0.1% (=1 g/l) 1.037, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 42.02
This classifies the protein as unstable.
Aliphatic index: 65.25
Grand average of hydropathicity (GRAVY): -0.708