ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MYRDAGAKTK KHRRSEETQR WKVCRHCHEK QDSTFFSIHS NYKMSNNETE TTPSRIVGFY 

        70         80         90        100        110        120 
ADWERTDITS HQVAKLTHAV FAYVQMKFDG SLGFKNDAAR IRFSNLRDKV RRNEDSNVKM 

       130        140        150        160        170        180 
MISIGGFENS QHFYPVLSNV EMKKAFLNSI SSFLAYHELH GVDIFWKWPS PEDKAHYSRF 

       190        200        210        220        230        240 
LADLRQHLGY EFIISVAVPQ AEVSNLELGY DLRTISSHVD FFNVHSMDYY GPWPNEWGKP 

       250        260        270        280        290        300 
TGPISPLYGP TRHNVDWTLR YYAEKTGEPG KLNMVIPFFV RLWKNVPEPV EPGRQVFRDV 

       310        320        330        340        350        360 
ELVDNKPQGE AYMSRWSAQH EELDLSPADW DEETRSSYTW NPDTRNFVTF ETDKSIQEKM 

       370        380        390        400        410 
KYVKEKNLGG VWIWHVDANE KLLDSVRFDG EGVVDDQEYR EIGLDISSLS 

References and documentation are available.

Number of amino acids: 410

Molecular weight: 47910.63

Theoretical pI: 5.99

Amino acid composition: 
Ala (A)  19	  4.6%
Arg (R)  25	  6.1%
Asn (N)  21	  5.1%
Asp (D)  28	  6.8%
Cys (C)   2	  0.5%
Gln (Q)  12	  2.9%
Glu (E)  32	  7.8%
Gly (G)  22	  5.4%
His (H)  16	  3.9%
Ile (I)  17	  4.1%
Leu (L)  25	  6.1%
Lys (K)  26	  6.3%
Met (M)  10	  2.4%
Phe (F)  22	  5.4%
Pro (P)  19	  4.6%
Ser (S)  34	  8.3%
Thr (T)  19	  4.6%
Trp (W)  13	  3.2%
Tyr (Y)  18	  4.4%
Val (V)  30	  7.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 60
Total number of positively charged residues (Arg + Lys): 51

Atomic composition:

Carbon      C	      2153
Hydrogen    H	      3231
Nitrogen    N	       589
Oxygen      O	       635
Sulfur      S	        12

Formula: C2153H3231N589O635S12
Total number of atoms: 6620

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    98445
Abs 0.1% (=1 g/l)   2.055, assuming all pairs of Cys residues form cystines


Ext. coefficient    98320
Abs 0.1% (=1 g/l)   2.052, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.63
This classifies the protein as unstable.



Aliphatic index: 65.80

Grand average of hydropathicity (GRAVY): -0.703