ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MYRPNFYEST CLRCSETVYQ VDRVGPLKDF TFFHSGCFKC VHCGTKLTLK TYFNNQHKQD 

        70         80         90        100        110        120 
DKEVYCSSHV PKSGPGHLDQ TSVGIRQALN APRTNKFVNE QIRGTRSEVD GGPLGGSRQS 

       130        140        150        160        170        180 
TPNGYGSREI SSPSQNDSDY KYGRFDASAL HIAHALKQTE IQKAYNKARE KPIDFYLARE 

       190        200        210        220        230        240 
EQAHLEMKHR KEEDDLYRKF ASKRAEEDRK IQDEFQDEWE RELQRLTHKF EKELATSRRS 

       250        260        270        280        290        300 
RDEANILTMR HEQQKEDLEK NMTLRRSKKK ESITRKMLEH ERYETAALVD RQSSEMLELI 

       310        320        330        340        350        360 
SARRSEYMQS ESIFLDDEFS EGAVPVEYPL NAPIPAPPAV SKFQIYTDPI EFEDVDRIAI 

       370        380        390        400        410        420 
SVAQEDQKTF TDLVRQLVGR CTTDIEKART IFRWITVKNL NAMHFDDDLR GDTPMGLLRG 

       430        440        450        460        470        480 
IKYGTESYHV LFKRLCSYAG LHCVVIKGYS KSAGYQPGVK FQDSRFRNSW NAVYVAGAWR 

       490        500        510        520        530        540 
FVQCNWGARH LVNAKEAPKQ GRGKNDSLRY EYDDHYFLTD PREFIYEFYP LQEEWQLLKR 

       550        560        570        580        590        600 
PITLREFENL PFVRSLFFRY GLHFADEGYG AVVFTDDTGA ATVRIAMPTD MQSCLIFHYN 

       610        620        630        640        650        660 
LKFYDSDEEL SYDGVSLKRF VMQSVIGNIV AFRVHAPCSG AFLLDIFANA VTPQEYLTGE 

       670        680        690        700        710        720 
PMKFKSVCKF KICCEELQTV MVPLPDCASG EWGPTKATRL FGLIPITHQD PLIFAGRSLD 

       730        740        750        760        770        780 
LQFRMSRPLT DFMATLHKNG IEEKKLAKYV THSTLDDIVT FIINFPEEGQ YGLDIYTREL 

       790        800        810 
GGPQHHHQHN NNNSSSSGEK HLLTHCCKYL INSSKRN 

References and documentation are available.

Number of amino acids: 817

Molecular weight: 94368.61

Theoretical pI: 6.87

Amino acid composition: 
Ala (A)  48	  5.9%
Arg (R)  57	  7.0%
Asn (N)  31	  3.8%
Asp (D)  50	  6.1%
Cys (C)  18	  2.2%
Gln (Q)  37	  4.5%
Glu (E)  63	  7.7%
Gly (G)  46	  5.6%
His (H)  29	  3.5%
Ile (I)  38	  4.7%
Leu (L)  66	  8.1%
Lys (K)  53	  6.5%
Met (M)  16	  2.0%
Phe (F)  46	  5.6%
Pro (P)  35	  4.3%
Ser (S)  55	  6.7%
Thr (T)  45	  5.5%
Trp (W)   7	  0.9%
Tyr (Y)  36	  4.4%
Val (V)  41	  5.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 113
Total number of positively charged residues (Arg + Lys): 110

Atomic composition:

Carbon      C	      4192
Hydrogen    H	      6466
Nitrogen    N	      1174
Oxygen      O	      1248
Sulfur      S	        34

Formula: C4192H6466N1174O1248S34
Total number of atoms: 13114

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    93265
Abs 0.1% (=1 g/l)   0.988, assuming all pairs of Cys residues form cystines


Ext. coefficient    92140
Abs 0.1% (=1 g/l)   0.976, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.66
This classifies the protein as unstable.



Aliphatic index: 70.07

Grand average of hydropathicity (GRAVY): -0.622