ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MYSTPLKKRI DYDRETFTAS ASLGGNRLRN RPRDDQNNGK PNLSSRSFLS ERKTRKDVLN 

        70         80         90        100        110        120 
KYGEAGNTIE SELRDVTTHV KISGLTSSEP LQLASEFVQD LSFRDRNTPI LDNPDYYSKG 

       130        140        150        160        170        180 
LDYNFSDEVG GLGAFTPFQR QQVTNIPDEV LSQVSNTEIK SDMGIFLELN YCWITSDNKL 

       190        200        210        220        230        240 
ILWNINNSSE YHCIDEIEHT ILKVKLVKPS PNTFVSSVEN LLIVATLFDI YILTISFNDR 

       250        260        270        280        290        300 
THELNIFNTG LKVNVTGFNV SNIISYERTG QIFFTGATDG VNVWELQYNC SENLFNSKSN 

       310        320        330        340        350        360 
KICLTKSNLA NLLPTKLIPS IPGGKLIQKV LEGDAGTEEE TISQLEVDQS RGVLHTLSTK 

       370        380        390        400        410        420 
SIVRSYLITS NGLVGPVLID AAHIRRGMNA LGVKNSPLLS NRAFKIAKIV SISMCENNDL 

       430        440        450        460        470        480 
FLAVITTTGV RLYFKGSISR RSIGSLKLDS VKFPPTSISS SLEQNKSFII GHHPLNTHDT 

       490        500        510        520        530        540 
GPLSTQKASS TYINTTCAST IISPGIYFTC VRKRANSGEL SKGITNKALL ENKEEHKLYV 

       550        560        570        580        590        600 
SAPDYGILKN YGKYVENTAL LDTTDEIKEI VPLTRSFNYT STPQGYANVF ASQYSAEPLK 

       610        620        630        640        650        660 
VAVLTSNALE IYCYRTPDEV FESLIENPLP FIHSYGLSEA CSTALYLACK FNKSEHIKSS 

       670        680        690        700        710        720 
ALAFFSAGIP GVVEIKPKSS RESGSVPPIS QNLFDKSGEC DGIVLSPRFY GSALLITRLF 

       730        740        750        760        770        780 
SQIWEERVFV FKRASKTEKM DAFGISITRP QVEYYLSSIS VLADFFNIHR PSFVSFVPPK 

       790        800        810        820        830        840 
GSNAITASDA ESIAMNALIL LINSIKDALS LINVFYEDID AFKSLLNTLM GAGGVYDSKT 

       850        860        870        880        890        900 
REYFFDLKFH DLFTPNAKTK QLIKEILIEV VNANIASGTS ADYIVNVLKE RFGSFCHSAD 

       910        920        930        940        950        960 
ILCYRAGEHL EAAQKFEMID SKISRNHLDT AIDLYERCAE NIELCELRRV VDIMVKLNYQ 

       970        980        990       1000       1010       1020 
PKTVGFLLRF ADKIDKGNQA QEYVSRGCNT ADPRKVFYDK RINVYTLIFE IVKSVDDYTS 

      1030       1040       1050       1060       1070       1080 
IEQSPSIANI SIFSPASSLK KRVYSVIMNS NNRFFHYCFY DWLVANKRQD YLLRLDSQFV 

      1090       1100       1110       1120       1130       1140 
LPYLKERAEK SLEISNLLWF YLFKEEHFLE AADVLYALAS SDFDLKLSER IECLARANGL 

      1150       1160       1170       1180       1190       1200 
CDSSTSFDQK PALVQLSENI HELFDIASIQ DDLLNLVRNE TRIDEDYRKQ LTLKLNGRVL 

      1210       1220       1230       1240       1250       1260 
PLSDLFNDCA DPLDYYEIKL RIFKVSQFKD EKVIQGEWNR LLDSMKNAPS PDVGSVGQES 

      1270       1280       1290       1300       1310       1320 
FLSSISNTLI RIGKTTRDTD VVFPVHFLMN KILESFIDKS SAADGSVCSM FLLAGVSHLK 

      1330       1340       1350       1360       1370       1380 
LYYILSRIIE NSEGNVELAK KEMVWLIKDW YQSDSDLRGS IAPEQIKKLE KYDPNTDPVQ 

      1390 
DYVKDRHHGL K 

References and documentation are available.

Number of amino acids: 1391

Molecular weight: 156649.30

Theoretical pI: 6.04

Amino acid composition: 
Ala (A)  76	  5.5%
Arg (R)  63	  4.5%
Asn (N)  87	  6.3%
Asp (D)  83	  6.0%
Cys (C)  21	  1.5%
Gln (Q)  37	  2.7%
Glu (E)  86	  6.2%
Gly (G)  65	  4.7%
His (H)  24	  1.7%
Ile (I) 108	  7.8%
Leu (L) 148	 10.6%
Lys (K)  92	  6.6%
Met (M)  14	  1.0%
Phe (F)  71	  5.1%
Pro (P)  51	  3.7%
Ser (S) 144	 10.4%
Thr (T)  74	  5.3%
Trp (W)   9	  0.6%
Tyr (Y)  55	  4.0%
Val (V)  83	  6.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 169
Total number of positively charged residues (Arg + Lys): 155

Atomic composition:

Carbon      C	      7027
Hydrogen    H	     11053
Nitrogen    N	      1853
Oxygen      O	      2127
Sulfur      S	        35

Formula: C7027H11053N1853O2127S35
Total number of atoms: 22095

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   132700
Abs 0.1% (=1 g/l)   0.847, assuming all pairs of Cys residues form cystines


Ext. coefficient   131450
Abs 0.1% (=1 g/l)   0.839, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.39
This classifies the protein as stable.



Aliphatic index: 94.54

Grand average of hydropathicity (GRAVY): -0.206