ProtParam
User-provided sequence:
10 20 30 40 50 60
MYTNYSLTSS DAMPRTYLVG TASPEMSKKK RQSANCDKPT RRVIHIIDTN EHSEVDLKNE
70 80 90 100 110 120
LPITCTNEDG EMTSSSWTSQ TANDFLKLAY VNAKLDPSLP SQYFKQDIIN VLQSLEIPGW
130 140 150 160 170 180
SVPGSKESSL NKNLLTLTQI KGALTNVIYK IHYPNLPPLL MRIFGDSIDS VIDREYELKV
190 200 210 220 230 240
IARLSFYDLG PKLEGFFENG RFEKYIEGSR TSTQADFIDR DTSIKIAKKL KELHCTVPLT
250 260 270 280 290 300
HKEITDQPSC WTTFDQWIKL IDSHKEWVSN NVNISENLRC SSWNFFLKSF KNYKRWLYND
310 320 330 340 350 360
SAFTSKLLRE DDKDSMINSG LKMVFCHNDL QHGNLLFKSK GKDDISVGDL TIIDFEYAGP
370 380 390 400 410 420
NPVVFDLSNH LNEWMQDYND VQSFKSHIDK YPKEEDILVF AQSYINHMNE NHVKIASQEV
430 440 450 460 470 480
RILYNLIIEW RPCTQLFWCL WALLQSGRLP QRPLIEGEKL MSEKAGLGDE THLMEHKNKE
490 500 510 520 530
NGKYDCSEDD SFNYLGFCKE KMSVFWGDLI TLGVIDKDCP DIGKTDYLDT KLIF
References and
documentation are available.
Number of amino acids: 534
Molecular weight: 61656.99
Theoretical pI: 5.55
Total number of negatively charged residues (Asp + Glu): 76
Total number of positively charged residues (Arg + Lys): 62
Atomic composition:
Carbon C 2762
Hydrogen H 4269
Nitrogen N 721
Oxygen O 834
Sulfur S 23
Formula: C
2762H
4269N
721O
834S
23
Total number of atoms: 8609
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 96425
Abs 0.1% (=1 g/l) 1.564, assuming all pairs of Cys residues form cystines
Ext. coefficient 95800
Abs 0.1% (=1 g/l) 1.554, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 46.83
This classifies the protein as unstable.
Aliphatic index: 82.87
Grand average of hydropathicity (GRAVY): -0.488