ProtParam

User-provided sequence:

        10         20         30 
NKQETHPRRK RFSAFGGDSP FGKQEAYVKL E 

References and documentation are available.

Number of amino acids: 31

Molecular weight: 3609.02

Theoretical pI: 9.82

Amino acid composition: 
Ala (A)   2	  6.5%
Arg (R)   3	  9.7%
Asn (N)   1	  3.2%
Asp (D)   1	  3.2%
Cys (C)   0	  0.0%
Gln (Q)   2	  6.5%
Glu (E)   3	  9.7%
Gly (G)   3	  9.7%
His (H)   1	  3.2%
Ile (I)   0	  0.0%
Leu (L)   1	  3.2%
Lys (K)   4	 12.9%
Met (M)   0	  0.0%
Phe (F)   3	  9.7%
Pro (P)   2	  6.5%
Ser (S)   2	  6.5%
Thr (T)   1	  3.2%
Trp (W)   0	  0.0%
Tyr (Y)   1	  3.2%
Val (V)   1	  3.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 4
Total number of positively charged residues (Arg + Lys): 7

Atomic composition:

Carbon      C	       160
Hydrogen    H	       247
Nitrogen    N	        49
Oxygen      O	        47
Sulfur      S	         0

Formula: C160H247N49O47
Total number of atoms: 503

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     1490
Abs 0.1% (=1 g/l)   0.413

Estimated half-life:

The N-terminal of the sequence considered is N (Asn).

The estimated half-life is: 1.4 hours (mammalian reticulocytes, in vitro).
                            3 min (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 94.83
This classifies the protein as unstable.



Aliphatic index: 28.39

Grand average of hydropathicity (GRAVY): -1.445