ProtParam
User-provided sequence:
10 20 30 40 50 60
PSWSKLTANS QYVSEYTPRG TGLACGTCVL SSDFGFILDC NFKKSGLFRV RNLGGLKAHF
70 80 90 100 110 120
GLGFSLGDEL GFPESLGNTE SDRRRAISYK NGAPPDLVGV LPSTQQQVNP PVQHPLPEKR
130 140 150 160 170 180
ISSRSTGAAP YNLQQVQQQQ QLQLQQQKLQ QQQLQQQLKL KQLLLLQQQQ QQQQQLMTEA
190 200 210 220 230 240
RPLPLGVPAF RPIPQKQLAA TQPSAPSSTP AERRVAVDTP EPRVSVNNKL VSSIDQPHSN
250 260 270 280 290 300
RTFHVANPVV SQPVAVPVFQ AMPQSIARIA NVGKEHRTVS SHPNYLQFEE PKFDLKDVTV
310 320 330 340 350 360
EELAAAANVT VDTIKHAIYV REQQLKAEHR AQLAAKLREE FMRTSTVRTT TTTTTTTTTT
370 380 390 400 410 420
PRPQKSLAEH KVSKVMNAPK EYYPVGYDKN FDDNFKSKVD LPPTSFSCAK QKHFPGLYAD
430 440 450 460 470 480
TDLGCMVFHV CALTDDGMVR KSFLCPENTL FDQTILKCNW WFYVDCSSST SVYDSNIPIS
490 500 510 520 530 540
KSYQLMKSLT YFSKYAGGQR HEQGGDKDEN ALDIDSLRES MEGVARRQEQ AKKAELRVEE
550 560
QRSMPKEDHE HVVPAEAEQQ LSN
References and
documentation are available.
Number of amino acids: 563
Molecular weight: 63042.16
Theoretical pI: 8.69
Total number of negatively charged residues (Asp + Glu): 58
Total number of positively charged residues (Arg + Lys): 64
Atomic composition:
Carbon C 2769
Hydrogen H 4385
Nitrogen N 793
Oxygen O 855
Sulfur S 18
Formula: C
2769H
4385N
793O
855S
18
Total number of atoms: 8820
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 39350
Abs 0.1% (=1 g/l) 0.624, assuming all pairs of Cys residues form cystines
Ext. coefficient 38850
Abs 0.1% (=1 g/l) 0.616, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is P (Pro).
The estimated half-life is: >20 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
? (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 55.75
This classifies the protein as unstable.
Aliphatic index: 71.87
Grand average of hydropathicity (GRAVY): -0.618