ProtParam
User-provided sequence:
10 20 30 40 50 60
RDMDDEESWI KEKKLLVGSE DYGRDLTGVQ NLRKKHKRLE GRLAEPEQAI QGVLDSGKKL
70 80 90 100 110 120
IDDNTIGREE IQQRPEQFVE HWKELKQLAA ARGQRLEESL EYQQFVANVE EEEAWINEKM
130 140 150 160 170 180
TLVASEDYGD TLAAIQGLLK KHEAFETDFT VHKDRVNDVC TNGQDLIKKN NHHEENISSK
190 200 210 220 230 240
MKGLNGKVSD LEKAAAQRKA KLDENSAFLQ FNWKADVVES WIGEKENSLK TDDYGRDLSS
250 260 270 280 290 300
VQTLLTKQET FDAGLQAFQQ EGIANITALK DQLLAAKHIQ SKAIEARHAS LMKRWTQLFA
310 320 330 340 350 360
NSATRKKKLL EAQSHFRKVE DLFLAFAKKA SAFNSWFENA EEDLTDPVRC NSLEEIKALR
370 380 390 400 410 420
EAHDAFRSSL SSAQADFNQL AELDRQIKSF RVASNPYTWF TMEALEETWR NLQKIIKERE
430 440 450 460 470
LELQKEQRRQ EENDKLRQEF AQHANAFHQW IQETRTYLLD GSCIGRRVGN SGISA
References and
documentation are available.
Number of amino acids: 475
Molecular weight: 54713.21
Theoretical pI: 5.65
Total number of negatively charged residues (Asp + Glu): 83
Total number of positively charged residues (Arg + Lys): 71
Atomic composition:
Carbon C 2398
Hydrogen H 3796
Nitrogen N 696
Oxygen O 755
Sulfur S 8
Formula: C
2398H
3796N
696O
755S
8
Total number of atoms: 7653
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 64065
Abs 0.1% (=1 g/l) 1.171, assuming all pairs of Cys residues form cystines
Ext. coefficient 63940
Abs 0.1% (=1 g/l) 1.169, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is R (Arg).
The estimated half-life is: 1 hours (mammalian reticulocytes, in vitro).
2 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 42.38
This classifies the protein as unstable.
Aliphatic index: 77.33
Grand average of hydropathicity (GRAVY): -0.814