ProtParam

User-provided sequence:

        10         20         30         40         50         60 
SKAVGIDLGT TYSCVAHFAN DRVEIIANDQ GNRTTPSYVA FTDTERLIGD AAKNQAAMNP 

        70         80         90        100        110        120 
HNTVFDAKRL IGRKFDDPEV TNDAKHYPFK VIDKGGKPVV QVEYKGETKT FTPEEISSMI 

       130        140        150        160        170        180 
LTKMKETAEN FLGTEVKDAV VTVPAYFNDS QRQATKDAGT IAGLNVLRII NEPTAAAIAY 

       190        200        210        220        230        240 
GLDKKSQKEH NVLIFDLGGG TFDVSLLSID EGVFEVKATA GDTHLGGEDF DSRLVNFLAE 

       250        260        270        280        290        300 
EFKRKNKKDL TTNQRSLRRL RTAAERAKRT LSSSAQTSIE IDSLFEGIDF YTSITRARFE 

       310        320        330        340        350        360 
ELCADLFRST LEPVEKVLAD SKLDKSQIDE IVLVGGSTRI PKVQKLVSDF FNGKEPNRSI 

       370        380        390        400        410        420 
NPDEAVAYGA AVQAAILTGD QSSTTQDLLL LDVAPLSLGI ETAGGIMTKL IPRNSTIPTK 

       430        440        450        460        470        480 
KSEVFSTYAD NQPGVLIQVF EGERTRTKDN NLLGKFELSG IPPAPRGVPQ IEVTFDIDAN 

       490        500        510        520        530        540 
GILNVSAVEK GTGKSNKITI TNDKGRLSKE DIDKMVAEAE KFKAEDEQEA QRVQAKNQLE 

       550        560        570        580        590        600 
SYAFTLKNSV SENNFKEKVG EEDARKLEAA AQDAINWLDA SQAASTEEYK ERQKELEGVA 

       610        620        630        640 
NPIMSKFYGA AGGAPGAGPV PGAGAGPTGA PDNGPTVEEV D 

References and documentation are available.

Number of amino acids: 641

Molecular weight: 69519.89

Theoretical pI: 5.02

Amino acid composition: 
Ala (A)  65	 10.1%
Arg (R)  28	  4.4%
Asn (N)  33	  5.1%
Asp (D)  45	  7.0%
Cys (C)   2	  0.3%
Gln (Q)  23	  3.6%
Glu (E)  53	  8.3%
Gly (G)  49	  7.6%
His (H)   5	  0.8%
Ile (I)  37	  5.8%
Leu (L)  46	  7.2%
Lys (K)  50	  7.8%
Met (M)   6	  0.9%
Phe (F)  28	  4.4%
Pro (P)  27	  4.2%
Ser (S)  39	  6.1%
Thr (T)  48	  7.5%
Trp (W)   1	  0.2%
Tyr (Y)  12	  1.9%
Val (V)  44	  6.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 98
Total number of positively charged residues (Arg + Lys): 78

Atomic composition:

Carbon      C	      3052
Hydrogen    H	      4886
Nitrogen    N	       842
Oxygen      O	       993
Sulfur      S	         8

Formula: C3052H4886N842O993S8
Total number of atoms: 9781

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    23505
Abs 0.1% (=1 g/l)   0.338, assuming all pairs of Cys residues form cystines


Ext. coefficient    23380
Abs 0.1% (=1 g/l)   0.336, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is S (Ser).

The estimated half-life is: 1.9 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 32.99
This classifies the protein as stable.



Aliphatic index: 80.55

Grand average of hydropathicity (GRAVY): -0.440