ProtParam

User-provided sequence:

        10         20         30         40         50         60 
SKAVGIDLGT TYSCVAHFSN DRVDIIANDQ GNRTTPSFVG FTDTERLIGD AAKNQAAMNP 

        70         80         90        100        110        120 
ANTVFDAKRL IGRNFNDPEV QGDMKHFPFK LIDVDGKPQI QVEFKGETKN FTPEQISSMV 

       130        140        150        160        170        180 
LGKMKETAES YLGAKVNDAV VTVPAYFNDS QRQATKDAGT IAGLNVLRII NEPTAAAIAY 

       190        200        210        220        230        240 
GLDKKGKEEH VLIFDLGGGT FDVSLLSIED GIFEVKATAG DTHLGGEDFD NRLVNHFIQE 

       250        260        270        280        290        300 
FKRKNKKDLS TNQRALRRLR TACERAKRTL SSSAQTSVEI DSLFEGIDFY TSITRARFEE 

       310        320        330        340        350        360 
LCADLFRSTL DPVEKVLRDA KLDKSQVDEI VLVGGSTRIP KVQKLVTDYF NGKEPNRSIN 

       370        380        390        400        410        420 
PDEAVAYGAA VQAAILTGDE SSKTQDLLLL DVAPLSLGIE TAGGVMTKLI PRNSTIPTKK 

       430        440        450        460        470        480 
SEVFSTYADN QPGVLIQVFE GERAKTKDNN LLGKFELSGI PPAPRGVPQI EVTFDVDSNG 

       490        500        510        520        530        540 
ILNVSAVEKG TGKSNKITIT NDKGRLSKED IEKMVAEAEK FKEEDEKESQ RIASKNQLES 

       550        560        570        580        590        600 
IAYSLKNTIS EAGDKLEQAD KDAVTKKAEE TIAWLDSNTT ATKEEFDDQL KELQEVANPI 

       610        620        630 
MSKLYQAGGA PEGAAPGGFP GGAPPAPEAE GPTVEEVD 

References and documentation are available.

Number of amino acids: 638

Molecular weight: 69338.81

Theoretical pI: 4.94

Amino acid composition: 
Ala (A)  59	  9.2%
Arg (R)  26	  4.1%
Asn (N)  32	  5.0%
Asp (D)  48	  7.5%
Cys (C)   3	  0.5%
Gln (Q)  24	  3.8%
Glu (E)  53	  8.3%
Gly (G)  49	  7.7%
His (H)   5	  0.8%
Ile (I)  38	  6.0%
Leu (L)  48	  7.5%
Lys (K)  52	  8.2%
Met (M)   7	  1.1%
Phe (F)  28	  4.4%
Pro (P)  28	  4.4%
Ser (S)  39	  6.1%
Thr (T)  45	  7.1%
Trp (W)   1	  0.2%
Tyr (Y)  10	  1.6%
Val (V)  43	  6.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 101
Total number of positively charged residues (Arg + Lys): 78

Atomic composition:

Carbon      C	      3043
Hydrogen    H	      4879
Nitrogen    N	       835
Oxygen      O	       991
Sulfur      S	        10

Formula: C3043H4879N835O991S10
Total number of atoms: 9758

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    20525
Abs 0.1% (=1 g/l)   0.296, assuming all pairs of Cys residues form cystines


Ext. coefficient    20400
Abs 0.1% (=1 g/l)   0.294, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is S (Ser).

The estimated half-life is: 1.9 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 31.76
This classifies the protein as stable.



Aliphatic index: 81.36

Grand average of hydropathicity (GRAVY): -0.450