ProtParam

User-provided sequence:

        10         20         30         40         50         60 
SLVISGLRHF TGYRIELQAC NQDSPEERCS VAAYVSARTM PEAKADDIVG PVTHEIFENN 


VV 

References and documentation are available.

Number of amino acids: 62

Molecular weight: 6835.66

Theoretical pI: 4.85

Amino acid composition: 
Ala (A)   6	  9.7%
Arg (R)   4	  6.5%
Asn (N)   3	  4.8%
Asp (D)   3	  4.8%
Cys (C)   2	  3.2%
Gln (Q)   2	  3.2%
Glu (E)   6	  9.7%
Gly (G)   3	  4.8%
His (H)   2	  3.2%
Ile (I)   4	  6.5%
Leu (L)   3	  4.8%
Lys (K)   1	  1.6%
Met (M)   1	  1.6%
Phe (F)   2	  3.2%
Pro (P)   3	  4.8%
Ser (S)   5	  8.1%
Thr (T)   3	  4.8%
Trp (W)   0	  0.0%
Tyr (Y)   2	  3.2%
Val (V)   7	 11.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 9
Total number of positively charged residues (Arg + Lys): 5

Atomic composition:

Carbon      C	       296
Hydrogen    H	       468
Nitrogen    N	        84
Oxygen      O	        96
Sulfur      S	         3

Formula: C296H468N84O96S3
Total number of atoms: 947

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     3105
Abs 0.1% (=1 g/l)   0.454, assuming all pairs of Cys residues form cystines


Ext. coefficient     2980
Abs 0.1% (=1 g/l)   0.436, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is S (Ser).

The estimated half-life is: 1.9 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 59.12
This classifies the protein as unstable.



Aliphatic index: 86.45

Grand average of hydropathicity (GRAVY): -0.160